灵芝孢子粉增强免疫力模型的建立及流式细胞术检测的研究  

Effect of ganoderma lucidum spore on immunoregulation of immunosuppression mice

作　　者：杨颖[1] 李志[1] 黄俊明[1] 蔡玟[1] 李文立[1] 黄健康[1] 

机构地区：[1]广东省疾病预防控制中心,广州510300

出　　处：《中国卫生检验杂志》2010年第6期1286-1288,共3页Chinese Journal of Health Laboratory Technology

基　　金：广东省科技计划项目(2007B030801001)

摘　　要：目的:探讨环磷酰胺建立的免疫低下动物模型应用于保健食品增强免疫力检验的研究,并试图应用流式细胞术研究小鼠自然杀伤细胞(NK)细胞分化抗原CD69+/NKG2D+和T淋巴细胞分化抗原CD69+/CD3+检测在免疫低下动物模型评价中的应用。方法:以环磷酰胺一次较大剂量造模、适时强化建立免疫低下模型,灌胃给予灵芝孢子粉,30 d后处死,取外周血和脾,分别检测小鼠外周血自然杀伤细胞(NK)亚群CD69+/NKG2D+和外周血T淋巴细胞CD69+/CD3+亚群,同时进行脾NK细胞活性测定、ConA诱导的小鼠脾淋巴细胞转化试验,观察环磷酰胺对外周血T淋巴细胞、NK细胞免疫功能的影响。结果:灵芝孢子粉可使环磷酰胺降低的小鼠外周血和脾的活化的NK细胞和T淋巴细胞比例及T淋巴细胞转化增殖能力、脾NK细胞活性增强。结论:灵芝孢子粉可有效增强小鼠细胞免疫功能,流式细胞术检测T淋巴细胞和NK细胞分化抗原在免疫调节功能评价中有较好应用价值。Objective：To explore the effects of ganoderma lucidum spore on the immunity of the immunosupp ressionmice with glucocorticiods（GC）.Methods：Different dosages of ganoderma lucidum spore were intragastriced to the immunosupp ressionmice with glucocorticiods,and the weight of the immune organs and the phagocytic function of the macrophages and neutrophile,the expression in of CD3^＋,CD4^＋,CD8^＋,CD49b^＋ and CD19^＋ in the blood cellwere measured;And the total complement activity,the content of the cytokine and the valence of hemolysin in the mice serum were detected.Results：The immune function of the GC immunosupp ression mice were remarkable effected with ganoderma lucidum spore,it could significantly increase the index of the thymus and sp leen,imp rove the valence of hemolysin and the phagocytic function of the macrophages and neutrophile of the mice.The total comp lement activity,the content of the cytokine in serum,and the quantity of T,B and NK cell in the mice blood were influenced by ganoderma lucidum spore.Conclusion：The results show that ganoderma lucidum spore remarkable effect on the immunity of the immunosupp ression mice with GC,and it can promote accommodation the immune function ofmouse.

关 键 词：保健食品 免疫低下 动物模型 CD69^+/CD3^^+ CD69+/NKG2D^+ 

分 类 号：R392[医药卫生—免疫学]