尿白蛋白HPLC法检测在原发性IgA肾病中的初步应用  

Measurement of Alb in urine by HPLC initial application in primary IgA nephropathy

作　　者：郑佳音[1] 陈筱菲[1] 杨建荣[1] 刘存丽[1] 袁谦[1] 李菊花[2] 

机构地区：[1]温州医学院附属第一医院实验诊断中心,浙江温州325000 [2]中国人民解放军第一八一中心医院,广西桂林541002

出　　处：《中国卫生检验杂志》2010年第6期1446-1447,1549,共3页Chinese Journal of Health Laboratory Technology

基　　金：温州市科学技术局(Y20070153)

摘　　要：目的:比较放射免疫法和高效液相色谱(HPLC)法检测尿白蛋白浓度的差异,探讨高效液相色谱(HPLC)法在原发性IgA肾病(IgAN)的预后和随访中的应用价值。方法:对经肾病理确诊为原发性IgAN患者41例行HPLC法尿白蛋白检测,与放射免疫法检测尿白蛋白结果比较分析。结果:在原发性IgA肾病患者尿中,存在免疫化学非反应性白蛋白。采用HPLC法能检出更多的尿白蛋白,是放射免疫法结果的1.1～5.1倍。结论:HPLC法比放射免疫法能检出更多的尿白蛋白,提示尿白蛋白高效液相色谱(HPLC)法检测可用于原发性IgAN的长期、动态随访,并有助于评估肾病预后。Objective：To compare radioimmunoassay with high performance liquid（HPLC） detection of urinary albumin concentration,to evaluate the role of albumin in urine by HPLC in follow-up and prognosis of primary IgA nephropath.Methods：A total of 41 patients with primary IgA nephropathy were studied.Urine albumin was analyzed in all patients by HPLC and by immunoassay.Result：It presents immunochemically non reactive urinary albumin in the urine of patients with primary IgA nephropathy.HPLC method used can be detected more urinary albumin is the results of radioimmunoassay of 1.1～5.1-fold.Conclusion：Measurement of Alb in urine by HPLC may be used as an dynamic indicator of follow-up and a marker of prognosis.

关 键 词：IGA肾病 HPLC 

分 类 号：O657.72[理学—分析化学]