免疫固定电泳在湖北病人脑脊液检测的临床调查研究  

Clinical investigation of examination of cerebrospinal fluid of Hubei patients using immune fixation electrophoresis techniques

作　　者：苗丽霞[1] 杨峻 朱果果[1] 邱艳艳[1] 刘永娟[1] 刘万红[1] 

机构地区：[1]武汉大学基础医学院,武汉430071 [2]武汉市第九医院检验科,武汉430081

出　　处：《中国卫生检验杂志》2010年第6期1467-1468,1553,共3页Chinese Journal of Health Laboratory Technology

基　　金：武汉大学自主创新基金(3081002)资助

摘　　要：目的:在收集的湖北病人脑脊液中运用免疫固定电泳技术进行蛋白检测,评价该技术的临床应用价值和潜力。方法:对脑脊液和血清标本同时进行琼脂糖凝胶电泳分离蛋白后,采用免疫固定技术,进行显色后使样本呈现出寡克隆带(oligoclonal bands,OCB)。结果:167例脑脊液和血液标本,经电泳免疫固定酶标显色后,出现血脑屏障受损病例6例,OCB病例10例。其中17例多发性硬化症病人中,有5例出现OCB,2例出现血脑屏障受损。结论:采用酶标记抗血清的免疫固定电泳技术,检测病人脑脊液无须浓缩,检测灵敏度高,也可检测内源性合成免疫球蛋白,能够为中枢神经系统疾病的诊断提供实验依据,在临床检测方面具有广阔的应用前景。Objective：To test the proteins of cerebrospinal fluid of Hubei patients and evaluate clinical significance and potential application of immune fixation electrophoresis techniques in examination of cerebrospinal fluid（CSF）.Methods：The samples of CSF and serum from 167 patients which were separated respectively by agarose gel electrophosis,were fixed with antibody（against human Ig） and colorized with TTF3.Oligoclonal bands（OB） were observed in the positive samples.Results：The results showed that there were the samples of 6 patients presented blood-brain barrier（BBB） impairment,the samples of 10 patients had oligoclonal band.In 17 patients with multiple sclerosis,there were 5 OB-positive patients of and 2 patients of BBB impairment.Conclusion：The examination of the patients′ samples for OB using immune fixation electrophoresis and enzyme labeled antibodies coloration technique can be strongly recommended to obtain support for the diagnosis of diseases and is a direct and reliable method in examination of endogenous Ig of CSF.The method is important for clinical usefulness.

关 键 词：免疫固定电泳 脑脊液 寡克隆带 

分 类 号：R446.14[医药卫生—诊断学]