高效液相色谱法测定复方甲苯咪唑片剂的含量  

HPLC Determination of Compound Mebendazole Tablets

作　　者：尹嵘 

机构地区：[1]湖南省财贸医院药剂科,长沙410002

出　　处：《中国医疗前沿》2010年第10期68-69,共2页China Healthcare Innovation

摘　　要：目的用高效液相色谱法测定复方甲苯咪唑片剂中甲苯咪唑和盐酸左旋咪唑的含量。方法采用高效液相色谱法,使用C18(200mm×4.6mm5μm)色谱柱,以甲醇-0.5%醋酸胺为流动相。线性梯度洗脱,流速1.0mL?min-1,检测波长227nm。结果甲苯咪唑在50～250μg?mL-1范围内线性较好,其线性方程为C=-1.9875+1.6975×10-4A(n=5,r=0.9998);盐酸左旋咪唑在12.5～62.5μg?mL-1范围内线性较好,其线性方程为C=-1.9658+1.6995×10-5A(n=5,r=0.9998)。甲苯咪唑的平均回收率为99.5%RSD=0.4%(n=9),盐酸左旋咪唑的平均回收率为98.6%RSD=0.5%(n=9)。结论高效液相色谱法快速简捷、可靠、准确度高、重现性好,可同时测定复方制剂中两种成分。Objective To establish the HPLC method for the determination of Mebendazole and Levamisole Hydrochloride in their compound Mebendazole Captopril tablets. Methods The HPLC separation was carried out on a Kromasil C18 （200mm×4.6mm 5μm） Column with methanol-0.5%Ammonium Acetate as the mobile phase,detected at 227nm,flow rate：1.0mL/min. Results Two good linearity were demonstrated by linear equation C=-1.9875＋1.6975×10^-4A（n=5,r=0.9999） of Mebendazole and C=-1.9658＋1.6995×10^-5A（n=5,r=0.9999） of Levamisole Hydrochloride. The average recoveries of Mebendazole and Levamisole Hydrochloride were 99.5% RSD=0.4%（n=9）,98.6% RSD=0.5%（n=9）.Conclusion The method is simple,reliable and accurate for simultaneous determination of Mebendazole and Levamisole Hydrochloride.

关 键 词：甲苯咪唑 盐酸左旋咪唑 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]