自体骨髓基质干细胞移植联合电刺激治疗脊髓损伤的实验研究  

The empirical study on the repair of spinal cord injury by transplantation of autogeneic mesenchymal stem cells and electro-stimulant therapy

作　　者：赵华[1] 陈允震[1] 刘海春[1] 谢斌[1] 陈剑[1] 

机构地区：[1]山东大学齐鲁医院,济南250012

出　　处：《中国矫形外科杂志》2010年第12期1019-1022,共4页Orthopedic Journal of China

基　　金：山东省科技厅攻关课题(编号:2004GG3202011)

摘　　要：[目的]探讨大鼠脊髓损伤(SCI)后自体骨髓基质干细胞(MSCs)移植联合电刺激治疗的效果。[方法]60只大鼠制备脊髓损伤模型,随机分成4组:MSCs移植组、电刺激组、MSCs移植联合电刺激组、对照组。应用BBB评分、SEP检测、DTI成像及组织切片评定恢复情况。[结果]BBB评分实验组高于对照组,组间比较差异有统计学意义(P<0.05)。SEP检测在移植后第7、8周差异有统计学意义(P<0.05)。DTI成像及组织切片观测在移植后第7、8周有明显的恢复。[结论]3种治疗都促进了脊髓损伤后的功能及组织病理学恢复,以MSCs移植联合电刺激治疗组效果最明显。[Objective]To evaluate the effects of transplantation of autogeneic mesenchymal stem cells（MSCs） with electro-stimulant therapy after spinal cord injury（SCI）.[Method]Sixty rats were randomly assigned into four groups：MSCs transplation group,electro-stimulant therapy group,MSCs transplation combined with electro-stimulant group and control group.[Result]Statistical differences showed in inter-group in BBB score after 4 weeks（P0.05）.Statistical differences showed in SEP after 7 and 8 weeks（P0.05）.DTI and slice observations of the spinal cords at 7,8 weeks after transplantation showed the recovery of spinal cord lesion.[Conclusion]The three methods have positive effects on behavioral outcome and histopathological assessment after SCI,which is most pronounced after MSCs trasplation and electro-stimulant therapy.

关 键 词：自体 骨髓基质干细胞 电刺激 修复 

分 类 号：R651.2[医药卫生—外科学]