盐碱与干旱胁迫对碱菀种子萌发和TvNHX1表达的影响  

Effects of saline-alkali and drought stress on seed germination and TvNHX1 expression of Tripolium vulgare

作　　者：魏佳丽[1] 崔继哲[1] 赵鹤翔[1] 弭晓菊[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025

出　　处：《应用生态学报》2010年第6期1389-1394,共6页Chinese Journal of Applied Ecology

基　　金：黑龙江省科技攻关计划项目(GA06B103-7)资助

摘　　要：通过施加不同浓度NaCl(40～400mmol·L-1)、NaHCO3(20～200mmol·L-1)和聚乙二醇(PEG,5%～30%),分析盐碱和干旱胁迫对盐生植物碱菀种子萌发、幼芽生长以及Na+/H+逆向转运蛋白基因TvNHX1表达的影响.结果表明:40～160mmol·L-1NaCl、20mmol·L-1NaHCO3和5%～10%PEG处理对碱菀种子的萌发没有不利影响;但当NaCl浓度达到240mmol·L-1时,碱菀种子发芽率、根长和芽长均显著降低(P<0.05);NaHCO3≥50mmol·L-1时,种子发芽率显著降低(P<0.05),浓度达到130mmol·L-1时,发芽势、根长和芽长显著降低;PEG≥15%时,种子的萌发出现显著的滞后效应.发芽期TvNHX1呈组成型表达,但160mmol·L-1NaCl、100mmol·L-1NaHCO3和10%PEG的胁迫诱导使TvNHX1的表达明显升高,NaCl和PEG胁迫下TvNHX1表达的变化与萌芽种子的表型变化同步,TvNHX1在碱菀耐逆境胁迫中可能发挥重要作用.By applying 40-400 mmol·L-1 NaCl,20-200 mmol·L-1 NaHCO3,and 5%-30% PEG,this paper studied the effects of saline-alkali and drought stress on the seed germination and Na＋/H＋ antiporter gene （TvNHX1） expression of Tripolium vulgare. 40-160 mmol·L-1 NaCl,20 mmol·L-1 NaHCO3,and 5%-10% PEG-6000 had less effects on the seed germination; while ≥240 mmol·L-1 NaCl decreased the seed germination rate,root length,and shoot length （P0.05）,≥50 mmol·L-1 NaHCO3 decreased the seed germination rate （P0.05）,130 mmol·L-1 NaHCO3 decreased the seed germination potential,root length,and shoot length （P0.05）,and ≥15% PEG delayed the seed germination. TvNHX1 transcripts were basically constitutive at germination stage,but their expression increased distinctly at 160 mmol·L -1 NaCl,100 mmol·L-1 NaHCO3,and 10% PEG. Under salt and drought stress,the expression of TvNHX1 changed in-phase with the phenotype change of germinated seeds,suggesting that TvNHX1 play important roles in the tolerance of T. vulgare against adversity stress.

关 键 词：碱菀 盐碱和干旱胁迫 种子发芽 Na+/H+逆向转运蛋白基因 

分 类 号：Q945.78[生物学—植物学]