Cloning and mRNA expression of macrophage migration inhibitory factor (MIF) gene of large yellow croaker (P seudosciaena crocea)  

作　　者：MAO Yong XU Bing SU Yongquan ZHANG Zhiwen DING Shaoxiong WANG Ding WANG Jun 

机构地区：[1]Fisheries College of Guangdong Ocean University, Zhanjiang 524025, China [2]State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China

出　　处：《Acta Oceanologica Sinica》2010年第3期63-73,共11页海洋学报（英文版）

基　　金：The National Natural Science Foundation of China under contract No. 40976096;the National Department Public Benefit Research Foundation of China under contract No. 200903029;the National High Technology Research and Development Program of China (863 Program) under contract No. 2006AA10A405

摘　　要：Mammalian macrophage migration inhibitory factor （MIF） plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homologue in fish septicemia. The authors have cloned the MIF homologue in large yellow croaker Pseudosciaena crocea （LycMIF） using RACE approach. The full-length cDNA of LycMIF was 634 bases and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. As demonstrated by RT-PCR and QRT-PCR assay, MIF mRNAs were constitutively expressed in 11 selected tissues and were abundant in brain and liver. Moreover, the LycMIF transcripts in the liver and head kidney were responsive to bacteria infection and could be significantly up-regulated. Our results provide the first direct evidence that fish MIF was implicated in pathogenesis of fish vibrosis and play an important role in response to bacteria infection.Mammalian macrophage migration inhibitory factor （MIF） plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homologue in fish septicemia. The authors have cloned the MIF homologue in large yellow croaker Pseudosciaena crocea （LycMIF） using RACE approach. The full-length cDNA of LycMIF was 634 bases and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. As demonstrated by RT-PCR and QRT-PCR assay, MIF mRNAs were constitutively expressed in 11 selected tissues and were abundant in brain and liver. Moreover, the LycMIF transcripts in the liver and head kidney were responsive to bacteria infection and could be significantly up-regulated. Our results provide the first direct evidence that fish MIF was implicated in pathogenesis of fish vibrosis and play an important role in response to bacteria infection.

关 键 词：macrophage migration inhibitory factor （MIF） VIBRIOSIS large yellow croaker mRNA expression 

分 类 号：Q785[生物学—分子生物学] Q51