小鼠子宫蜕膜化对uNK细胞分布及成熟性的影响  

The effect of mouse uterine decidualization on NK cell distribution and maturation

作　　者：张莉[1] 徐宏[1] 张保平[1] 彭婧[1] 况海斌[1] 

机构地区：[1]南昌大学医学院生理学教研室,南昌330006

出　　处：《重庆医科大学学报》2010年第6期810-812,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金(编号:30760071;30960118);江西省教育厅基金(编号:GJJ08106;09132)

摘　　要：目的:探讨子宫自然杀伤(Uterine natural killer,uNK)细胞在小鼠妊娠早期子宫的分布及其调节。方法:用uNK细胞特异性的DBA-lectin抗体,对正常妊娠、延缓着床和人工诱导蜕膜化子宫壁内uNK细胞进行免疫组织化学标记。结果:在胚胎着床前,DBA-lectin染色主要分布于一些血管内皮细胞。至妊娠第5d,胚胎着床以后,NK细胞分布于子宫的系膜侧基质中,主要是一些小的、未成熟的uNK细胞。至妊娠第7、8d时,NK细胞分布于子宫的系膜侧蜕膜中,且逐渐变大和成熟,胞浆颗粒变得越来越致密。在人工诱导蜕膜化模型中,uNK细胞在子宫内膜的分布及成熟性与正常妊娠过程基本相似。结论:在妊娠早期,小鼠uNK细胞分布及成熟性主要受子宫内膜蜕膜化的影响。Objective：To investigate the distribution and regulation of uterine NK cells in mouse uterus during early pregnancy. Methods：Using a unique uNK cell marker,Dolichos biflorus agglutinin （DBA） lectin,to localize NK cells in uterus of normal pregnancy,delayed implantation and artificially induced decidualization. Results：DBA staining is mainly distributed in some vascular endothelial cells during embryo pre-implantation. In D5 of pregnancy after embryo implantation,NK cells were distributed in the stroma of uterine mesometrial pole,with round,immature and small lymphocyte-like cells. During D7 and D8 of pregnancy,the immunostaining results showed that uNK cells were increasingly accumulated in the mesometrial pole of implantation sites,with their morphology changing from small round cells with scattered cytoplasm to large and mature cells with heavy cytoplasmic granules. In the artificially induced decidualization model,the distribution and maturity of NK cells are similar to those of the normal pregnancy in the mouse uteri. Conclusion：The distribution and maturation of uterine NK cells are dependent of uterine decidualization in mouse early pregnancy.

关 键 词：子宫自然杀伤细胞 子宫 妊娠早期 蜕膜化 

分 类 号：R446.12[医药卫生—诊断学]