多环芳烃类对人群血浆中单胺类神经递质的影响  

Effects of polycylic aromatic hydrocarbons on plasma monoamine neurotransmitters

作　　者：何丽君[1] 汤艳[2] 何志军 何平中 涂白杰[1] 

机构地区：[1]重庆医科大学公共卫生学院劳动卫生与环境卫生学教研室,重庆400016 [2]泸州医学院公共卫生系环境卫生与劳动卫生教研室,泸州6460003 [3]重钢劳动卫生研究所,重庆400080

出　　处：《重庆医科大学学报》2010年第6期819-821,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:30671744)

摘　　要：目的:探讨多环芳烃类(Polycylic aromatic hydrocarbons,PAHs)对接触人群血浆中单胺类神经递质的影响。方法:选取80名某钢铁公司焦化厂健康成年男性焦化作业工人作为接触组,80名氧气厂一般情况相近的工人作为对照组。使用高效液相-荧光测定法检测作业环境中苯并芘(Benzo[a]pyrene,B[a]P)浓度、高效液相-荧光测定法检测尿中1-羟基芘(1-hydrox ypyrene,1-OHP)水平、高效液相-电化学检测法测定血浆中去甲肾上腺素(Noradrenaline,NE)、肾上腺素(Adrenaline,AD)、多巴胺(Dopamine,DA)和五羟色胺(Serotonin,5-HT)含量。结果:作业环境中B[a]P浓度接触组明显高于对照组(P<0.05)。与对照组相比,接触组尿中1-OHP含量、血浆中NE、DA、5-HT含量明显增高(P<0.05),AD含量无明显变化,并且NE、DA、5-HT含量与尿中1-OHP含量存在回归关系(P<0.05)。结论:PAHs可致接触人群中单胺类神经递质升高的改变。Objective：To explore the effect of polycylic aromatic hydrocarbons （PAHs）on plasma monoamine neurotransmitters. Methods：80 healthy adult male coke oven workers were selected from a coke plant of a state-owned steel enterprise,80 controls were selected from the oxygen factory. Concentration of benzo[a]pyrene（B[a]）P in the working environment was monitored by high performance liquid chromatography（HPLC）with fluorescence detector. The level of urinary 1-hydroxypyrene（1-OHP）was determ ined by HPLC with fluorescence detector.The plasma noradrenaline（NE）,adrenaline（AD）,dopamine（DA）and serotonin（5-HT）levels were measured by high performance liquid chromatography （HPLC） with electrochemical detection. Results：In the working environment of B[a]P, concentration in coke oven workers were significantly higher than controls （P0.05）.The urinary 1-OHP concentrations in coke oven workers were significantly higher than controls （P〈0.05）.The plasma NE,DA,5-HT levels in coke oven workers were significantly higher than controls（P〈0.05）,the plasma A level have no significant difference,and results of correlation analysis showed that positive correlation between the plasma NE,DA,5-HT levels and the urinary 1-OHP concentrations. Conclusion：PAHs may cause an increase of plasma monoamine neurotransmitters in exposed population group.

关 键 词：多环芳烃 苯并芘 1-羟基芘 单胺类神经递质 

分 类 号：R135.99[医药卫生—劳动卫生]