纳米羟基磷灰石复合物修复骨缺损实验研究的影像学评价  

The study of radiology in the experiment of repairing bone defects with nano-hydroxyapatite and polyamide-66 composite

作　　者：肖智博[1] 吕富荣[1] 蒋电明[2] 欧阳羽[1] 吕发金[1] 李杰[2] 

机构地区：[1]重庆医科大学附属第一医院放射科,重庆400016 [2]重庆医科大学附属第一医院骨科,重庆400016

出　　处：《重庆医科大学学报》2010年第6期825-828,共4页Journal of Chongqing Medical University

基　　金：国家863计划子课题(编号:2002AA326020)

摘　　要：目的:通过磁共振成像(Magnetic resonance imaging,MRI)与X线方法检测纳米羟基磷灰石/聚酰胺(n-HA/PA66)复合材料应用于修复骨缺损实验研究,综合评价其成骨能力,为骨缺损修复后全面、准确的影像学评价提供理论依据。方法:30只新西兰大白兔行一侧胫骨髁钻孔,并植入n-HA/PA66复合材料。术后2、4、6、8、12周各时相点摄DR片、MRI及动态增强MRI检查,并取材进行组织学观察。结果:(1)MRI显示n-HA/PA66复合材料与骨组织易于区分,术后第4周开始从材料内部逐步强化,周围软组织水肿逐步吸收,术后12周修复区强化明显,周围水肿基本吸收。(2)X线显示术后第8周开始,缺损填修复区内的骨质增生开始,术后12周缺损区呈高密度骨质修复表现。(3)组织学显示术后4～12周,n-HA/PA66复合材料被界膜分隔成小岛,界膜内可见大量间充质细胞、成纤维细胞及小血管,后形成破骨及成骨细胞,修复区内骨小梁逐步形成。结论:MRI联合X线能够清楚显示n-HA/PA66复合材料修复过程,对早期骨移植的成功有很好预测作用,并兼顾术区周围关节及软组织的恢复显示,具有临床可操作性。Objective：Tostudy the MRI with X-ray in the ability of bony generation by the way of the experiment of repairing bone defects with n-HA/PA66. Methods：30 New Zealand rabbits were avalued whose condyle of tibia in one side was performed boring which was 3mm diameter and 5mm depth with n-HA/PA66 composite implanting. The specimens were taken respectively in different times：2nd 4th 6th 8th 12th week after surgery,and were examined by the methods of plain roentgenography、histology and magnetic resonance imaging. Results：（1）MRI and X-ray：From the 4th week,n-HA/PA66 composite had the poor defined border and the signal inter-material had become more variform,more obvious enhancement,at the same time in the X-ray,there were more and more bony generation（.2）The histology showed that a layer of interface membrane forming. From the 4th week,The interface membrane had been widest and the number of cells was increasing. Till the 12th week,there were some bone trabecula formed and the HA were separated into small islands,more osteoclasts appeared. Conclusion：The experimental results showthat the perfect radiology technique is useful to predict the bone transplantation. Furthermore it is easy to operate in clinical setting,and the n-HA/PA66 Composite has the attribute of good biocompatility and osteoconductibity,which can be manifested by plain roentgenography and magnetic resonance imaging.

关 键 词：骨移植 MRI 骨缺损 纳米羟基磷灰石-聚酰胺66复合物 

分 类 号：R816.8[医药卫生—放射医学]