HSPTX通过抑制NF-κB蛋白表达减轻失血性休克复苏诱发的肺部炎症反应  

Hypertonic saline and pentoxifylline reduces hemorrhagic shock resuscitation-induced pulmonary inflammation through inhibiting the expression of NF-κB

作　　者：刘玉[1] 唐季春[2] 张焰[1] 曾因明[2] 

机构地区：[1]无锡市第四人民医院麻醉科,无锡214062 [2]江苏省麻醉学重点实验室,徐州221002

出　　处：《重庆医科大学学报》2010年第6期877-880,共4页Journal of Chongqing Medical University

摘　　要：目的:探讨HSPTX(7%、5%氯化钠+己酮可可碱)对失血性休克大鼠肺损伤的保护作用。方法:24只雄性SD大鼠随机分为3组:假失血性休克(Sham)组,仅接受动静脉插管操作,不放血及复苏、大容量等张乳酸钠林格氏液(RL)复苏组,接受32ml/kgRL、小容量高张(7%、5%氯化钠)液+PTX复苏组,接受4ml/kg7.5%NaCl+25mg/kgPTX,每组8只。测定各组动脉血氧分压(PaO2)、pH值、二氧化碳分压(PaCO2)、肺湿/干重比值、测定血清丙二醛(Malondialdehyde)含量以及超氧化物歧化酶(Superoxide dismutase)活性;检测支气管肺泡灌洗液(Bronchoalveolor lavage fluid)中性粒细胞比例及肺通透性指数,采用ELISA法测定灌洗液上清中TNF-α、IL-1β含量;采用Western blot技术检测肺组织NF-κB蛋白表达。结果:与RL组相比,HSPTX组PaO2和pH值升高、PaCO2降低(P<0.01),HSPTX组大鼠肺湿/干重(W/D)、支气管肺泡灌洗液中上清中TNF-α、IL-1β含量均低于RL组(P<0.01),同时HSPTX组肺组织中核因子κB蛋白(NF-κB)表达显著低于RL组。结论:HSPTX复苏通过抑制核因子κB蛋白的表达减少失血性休克大鼠炎性细胞因子的表达,减轻由失血性休克诱发的急性肺损伤。Objective：To explore the effects of hypertonic saline and pentoxifylline resuscitation on pulmonary inflammation following hemorrhagic shock in rats. Methods：Controlled hemorrhagic shock was induced by blood withdrawal （MAP,40 mmHg）and maintained for 60 min. Animals were randomly divided into 3 groups：① Sham animals（n=8）underwent cannulation without shock or resuscitation and served as negative controls;②RL-resuscitated animals （n=8）received 32 ml/kg RL;③HSPTX group received 4 ml/kg of 7.5% NaCl＋25 mg/kg of PTX. PaO2,pH,PaCO2,wet/dry lung weight ratio（W/D）,and lung penetrating index were determined. The percentages of neutrophil in bronchoalveolar lavage fluid,the levels of malonadialdehyde （MDA）and superoxide dismutase （SOD）were measured. The tumor necrosis factor-α（TNF-α）and interleukin 1β（IL-1β）in the supernatant of bronchoalveolor lavage fluid were determined by ELISA method,and the expression of NF-κB in the lung tissues was quantified by Western blot. Results：PaO2 and pH were increased whereas PaCO2 was decreased in HSPTX group when compared with that in RL group （P〈0.01）. The wet/dry lung weight ratio and the percentages of neutrophil in the supernatant of bronchoalveolar lavage fluid were significantly lower than that in RL group（P〈0.01）. The content of tumor TNF-αand IL-1βin HSPTX group was significantly reduced when compared with that in RL group（P〈0.01）. The protein expression of NF-κB in HSPTX group was significantly decreased than that in RL group （P〈0.01）. Conclusion： Attenuation of hemorrhagic shock-induced pulmonary inflammation with HSPTX is associated with HSPTX-induced inhibition of neutrophil activation and production of inflammatory cytokines via inhibiting the expression of NF-κB.

关 键 词：己酮可可碱 高渗盐溶液 失血性休克 急性肺损伤 肿瘤坏死因子α 核因子ΚB 

分 类 号：R641[医药卫生—外科学]