牛皮下注射爱普菌素注射剂后组织残留预测模型研究  

Study on Prediction Model of Eprinomectin Residue in Bovine Tissues After Subcutaneous Administration

作　　者：江海洋[1] 吴聪明[1] 丁双阳[1] 赵思俊[2] 徐飞[1] 李建成[1] 夏曦[1] 刘金凤[1] 沈建忠[1] 

机构地区：[1]中国农业大学动物医学院/国家兽药残留基准实验室,北京100094 [2]中国动物卫生与流行病学中心,山东青岛266032

出　　处：《中国农业科学》2010年第13期2813-2819,共7页Scientia Agricultura Sinica

基　　金："十一五"国家科技支撑计划项目(2006BAD31B08);教育部新世纪优秀人才支持计划(NCET-08-0538)

摘　　要：【目的】本文研究是为了探讨动物活体样品(血液等)与组织(肌肉等)中药物残留量之间的关系,并建立相应的预测模型。【方法】牛颈部皮下一次性注射抗寄生虫药爱普菌素注射液(1%),注射剂量为0.5mg·kg-1体重,分别在给药后第1、第3、第7、第14、第21、第28、第42和第56天,各屠宰3头取样用高效液相色谱分析。【结果】采用SPSS统计学软件,以消除相血液中爱普菌素残留为自变量拟合的预测模型回归方程,R2均大于0.9,方程拟合度经F检验,P值均小于0.001;以消除相粪便中爱普菌素残留为自变量拟合的预测模型回归方程,R2均大于0.8,方程拟合度经F检验,P值均小于0.001,说明回归方程具有显著的统计学意义。【结论】牛皮下注射爱普菌素后,血液或粪便中爱普菌素残留与可食组织中的残留具有很强的相关性。【Objective】 The aim of this research is to find the relationship to eprinomectin （EPR） residue amount in bovine blood,urine,feces,hair and tissues including muscle,liver,kidney and fat and develop prediction models. 【Method】 Twenty-four parasite-free cross cattle were administered subcutaneously with 1% EPR injectable oil formulation at a dose of 0.5 mg·kg-1 body weight. Three treated animals （two males and one female） were selected randomly and killed at 1,3,7,14,21,28,42 and 56 days after injection. The samples mentioned above were collected and analyzed. 【Results】 The relative coefficients of prediction model regress equations fitted using SPSS 13.0 software with EPR residue concentration in blood in elimination phrase as independent variable were more than 0.9. The F test was used to analyze goodness of fit for equations with P values lower than 0.001. The relative coefficients of prediction model regress equations with EPR residue concentration in feces in elimination phrase as independent variable were more than 0.8. The F test used to analyze goodness of fit for equations with P values was lower than 0.001. 【Conclusion】 These results demonstrated that the prediction models had statistical significance and furthermore revealed that there is a strong correlation between amounts of EPR residue in blood or feces and edible tissues.

关 键 词：爱普菌素 牛 高效液相色谱 残留 预测模型 

分 类 号：S858.23[农业科学—临床兽医学]