HIV感染和抗反转录病毒治疗对PBMC线粒体DNA含量影响  

The effect of HIV infection and combination antiretroviral therapy on the mitochondrial DNA in PBMC from HIV/AIDS patients

作　　者：吴亚松[1] 陈新月[2] 石英[2] 张彤[2] 陈德喜[2] 张福杰[1,3] 吴昊[2] 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心治疗与关怀室,102206 [2]首都医科大学附属北京佑安医院感染科 [3]北京地坛医院

出　　处：《北京医学》2010年第6期431-435,共5页Beijing Medical Journal

基　　金：国家973项目(NO.2006CB504201)

摘　　要：目的探讨艾滋病病毒(HIV)和联合抗反转录病毒治疗(cART)对外周血单个核细胞(PBMC)中线粒体DNA含量的影响,及PBMC中线粒体DNA含量和脂肪代谢异常(LD)的关系。方法采用实时定量PCR检测未治疗、已经治疗的HIV感染者和正常对照组外周血单核细胞(PBMC)中线粒体DNA相对含量,比较不同人群PBMC中线粒体DNA有无差异,比较发生LD和未发生该症状患者线粒体DNA含量有无差异。结果未接受抗病毒治疗的HIV感染者和已治疗的HIV患者PBMC中线粒体DNA含量为非感染者的0.61倍和0.8倍,但无显著性差异(P=0.122)。发生LD患者PBMC中线粒体DNA相对含量(2-△△Ct)比无LD患者显著降低(0.57vs.1.12,P=0.045)。结论发生LD的患者血中PBMC中线粒体DNA含量显著下降,可能作为潜在临床辅助诊断指标,但需进一步研究。Objective To investigate the effects of HIV and combination antiretroviral therapy （cART） on mitochondrial DNA content in peripheral blood mononuclear cells （PBMCs） and the relationship between mitochondrial DNA content and lipodystrophy （LD）.Methods The mitochondrial DNA level in PBMCs from 20 HIV infected patients,40 ART experienced patients were determined using real-time PCR,with 20 healthy individuals serving as the normal controls.Results The mitochondrial DNA level in the PBMCs from patients with HIV was slightly lower than that in normal controls.The mtDNA content in PBMCs from patients with LD was significantly lower than that from patients without LD.Conclusions HIV infection and/or cART might lead to mitochondrial DNA in PBMC depletion.There is correlation between the amount of mitochondrial DNA in PBMC to LD but still need further evaluation.

关 键 词：艾滋病病毒 联合抗反转录病毒治疗 线粒体DNA 外周血单个核细胞 

分 类 号：R512.91[医药卫生—内科学]