丙烯菊酯和胺菊酯与DNA相互作用的光谱研究  

Studies on the Interaction of Allethrin and Tetramethrin with DNA by Means of Spectrometry

作　　者：吴朝阳[1] 刘引[1,2] 栾崇林[2] 蒋晓华[2] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室,湖南长沙410082 [2]深圳职业技术学院精细化工系,广东深圳518055

出　　处：《湖南大学学报（自然科学版）》2010年第6期61-66,共6页Journal of Hunan University:Natural Sciences

基　　金：湖南省科技计划资助项目(2008GK3151);深圳市科技计划资助项目(2108K037BC);深圳职业技术学院重点资助项目(2208K015BC)

摘　　要：采用紫外光谱和荧光光谱法,研究了丙烯菊酯和胺菊酯与单双链DNA、鸟嘌呤及鸟嘌呤核苷之间的相互作用.拟除虫菊酯农药可使DNA溶液的吸收光谱出现减色效应,但最大吸收波长无明显位移;不同浓度的DNA可导致农药荧光猝灭.还分别考察了离子强度、KI对农药-DNA体系的影响及农药对中性红-DNA体系的影响.结果表明:丙烯菊酯与ssDNA之间存在静电和沟槽两种作用方式,而胺菊酯与ssDNA之间主要是静电作用.鸟嘌呤和鸟嘌呤核苷与农药作用的紫外光谱和荧光光谱研究表明,它们与农药之间主要以氢键相互作用,且鸟嘌呤核苷与农药的作用比鸟嘌呤与农药的作用要强.The interaction of allethrin and tetramethrin with single strand DNA（ssDNA）,double strand DNA,guanine and guanosine was studied by means of UV and fluorescence spectrophotometry.Pyrethroid Pesticide brought about a hypochromic effect on the UV absorption spectrum of DNA,but the maximal absorption wavelength of DNA did not shift obviously and the addition of varied concentration of DNA caused the fluorescence quenching of pesticides.The effect of ionic strength and KI on the system of pesticide-DNA and the effect of pesticide on the neutral red-DNA were investigated.The results have indicated that the electrostatic binding and the groove binding are the two major modes of the interaction between allethrin and DNA,while electrostatic binding mainly exists between tetramethrin and DNA.The spectra of UV and the fluorescence of Guanine and guanosine interacting with pesticides have proved that the interaction between them and pesticide is chiefly hydrogen bonds,and guanosine interacts more strongly with pesticides than guanine does.

关 键 词：光谱法 丙烯菊酯 胺菊酯 DNA 作用方式 

分 类 号：O657.3[理学—分析化学]