银杏种仁中一种抗氧化活性蛋白的纯化及性质  

Purification and Characterization of an Antioxidant Active Protein from Ginkgo biloba Seed

作　　者：孟如杰[1] 田亚平[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,无锡214122

出　　处：《天然产物研究与开发》2010年第3期388-391,432,共5页Natural Product Research and Development

摘　　要：银杏种仁经破碎,提取缓冲液4℃浸取后离心得上清液。上清液经硫酸铵沉淀,DEAE-52离子交换层析,MonoQ离子交换层析,UltroGelACA-54凝胶过滤层析后,分离得到一种具有抗氧化活性的蛋白。该蛋白经UltroGelACA-54凝胶过滤层析测定分子量为60 kD,经PAGE和SDS-PAGE鉴定均为单一蛋白质条带。SDS-PAGE测定其亚基分子量为10 kD。该蛋白具有一定的还原能力和清除超氧阴离子自由基及DPPH自由基能力,并在30～60℃温度下具有良好的稳定性。An antioxidant active protein was isolated from the seeds of Ginkgo biloba by using the ammonium sulfate precipitation, the DEAE-52 and MonoQ ion-exchange chromatography, and the UltroGelACA-54 gel filtration chromatography. The protein gave a single band on PAGE and SDS-PAGE. The total molecular weight of the protein was 60 kD and the molecular weight of subunit was 10 kD. The protein showed the capability of reducing power, superoxide anion radical scavenging, and free radical scavenging activity, and was stable at the temperature of 30-60 ℃.

关 键 词：银杏 种仁蛋白 纯化 抗氧化 

分 类 号：R284.2[医药卫生—中药学] Q946[医药卫生—中医学]