当归内酯对免疫抑制小鼠免疫功能的重建作用(英文)  

Reconstitution of Immune Response by Angelica sinensis (Oliv.) Diels lactones in Immunocompromised Mice

作　　者：龙锐[1,2] 杨芳[2] 杜俊蓉[2] 王蓓[2] 

机构地区：[1]重庆医科大学附属第一医院药剂科,重庆400016 [2]四川大学华西药学院药理学与生物制药系,成都610041

出　　处：《天然产物研究与开发》2010年第3期398-402,共5页Natural Product Research and Development

摘　　要：探讨当归内酯(ASDL)对免疫抑制小鼠免疫功能的重构作用。通过小鼠腹腔注射环磷酰胺建立免疫抑制动物模型。采用免疫器官重量法和小鼠腹腔巨噬细胞吞噬鸡红细胞实验检测了ASDL对非特异性免疫功能的影响;用血清溶血素分光光度法检测了对体液免疫功能的作用;用MTT法进行了致分裂原诱导的小鼠脾淋巴细胞的增值反应实验,再用乳酸脱氢酶法测定了NK和CTL细胞活性,从而确定ASDL对小鼠细胞免疫功能的影响。结果表明:ASDL能够对免疫低下小鼠的非特性和特异性免疫功能起到一定的重构作用。但是这种效果并不是剂量依赖性的,20 mg/kg这个剂量的效果明显好于5和80 mg/kg这两个剂量。上述结果表明ASDL能够显著提高免疫低下小鼠的免疫功能。This paper studied the effects of lactone extracts from Angelica sinensis （Oliv. ） Diels （ASDL） on immunity functions in immunocompromised mice by different methods. The nonspecific immune function was measured by thymus, spleen index and macrophage phagocytosis. Determination of hemolysin concentration in serum by Quantitative hemolysis of sheep red blood cells assay revealed the humoral function. In addition, the proliferative response of lymphocyte to concanavalin And lipopolysaccharides, the activity of cytotoxic T lymphocyte and nature killer cells activity were studied to demonstrate the effects of ASDL on cellular immunogical functions. These effects were not dose-dependent in a linear fashion. A total of 20 mg/kg dose is more effective than 5 and 80mg/kg doses. In summary, we conelude that the oral treatment of immunocompromised mice with ASDL led to partial reconstitution of the nonspecific, humoral and cellular immune response.

关 键 词：当归 当归内酯 环磷酰胺 免疫抑制 小鼠 

分 类 号：R282.71[医药卫生—中药学] Q949[医药卫生—中医学]