拟心叶党参挥发油成分的GC-MS分析及其抗菌活性(英文)  

GC-MS Analysis and Antimicrobial Activity of Essential Oils from the Fresh and Dried Roots of Codonopsis cordifolioidea

作　　者：邱斌 吕青[2] 宝福凯[3] 张才军[3] 程永现[2] 

机构地区：[1]云南省药物研究所,昆明650111 [2]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,昆明650204 [3]昆明医学院微生物学与免疫学系,昆明650031

出　　处：《天然产物研究与开发》2010年第3期445-449,465,共6页Natural Product Research and Development

基　　金：supported by the "Xi-Bu-Zhi-Guang" Project from Chinese Academy of Sciences,;a Talent Scholarship for the Youth of Yunnan (No.2007PY01-48)

摘　　要：运用气-质联用技术对云南产桔梗科植物拟心叶党参(Codonopsis cordifolioidea)的鲜根和干根的挥发油成分进行了比较研究,鉴定了其中六十三个成分,并探讨了其具有浓郁臭味的分子基础。采用八种菌株对其挥发油成分进行了抗菌活性测试,结果表明仅金黄色葡萄球菌、伤寒沙门菌和痢疾杆菌为敏感菌株。The essential oils were extracted from the fresh and dried roots of Codonopsis cordifolioidea by steam distillation approaches,and sixty-three compounds were identified by GC-MS analysis. The main principles are linolic acid （ 21.9 % ）, retene （ 11.4 % ）, pentadecane （ 7.4 % ）, methyl-9,12,15-octadecatrienoate （ 6.8 % ）, and heneicosylcyclo- pentane （ 3.8% ）. The total oxygenated aromatic hydrocarbons reach to 8.8%. Some components such as 9,12,15-octadecatrienoic acid methyl ester in the essential oils of dried roots vary greatly compared to fresh materials. According to the different relative contents, the possible diagnostic molecular markers for the characteristic odor were pointed out. The antimicrobial activity of the both essential oils was evaluated against eight microbial strains using agar dilution method. The results showed that only Staphylococcus aureus, Salmonella typhimurium, and Shigella flexneri were sensitive to the essential oils.

关 键 词：拟心叶党参 桔梗科 气-质联用 挥发油 抗菌活性 

分 类 号：Q946.91[生物学—植物学] R284.1[医药卫生—中药学]