迭鞘石斛中性多糖DDP1-1的体内免疫活性  

In Vivo Immunomodulatory Activities of Neutral Polysaccharide (DDP1-1) from Dendrobium denneanum

作　　者：范益军[1] 淳泽[2] 罗傲雪[1] 罗傲霜[2] 何涛[2] 何兴金[3] 

机构地区：[1]四川农业大学都江堰校区,都江堰611830 [2]中国科学院成都生物研究所,成都610041 [3]四川大学生命科学院,成都610064

出　　处：《应用与环境生物学报》2010年第3期376-379,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家级星火计划项目(No.2008GA810003);四川省科技支撑计划项目(No.2010NZ0057);四川省科技平台建设项目;中国科学院成都生物研究所前沿项目资助~~

摘　　要：建立S180肉瘤模型,考察了迭鞘石斛中性多糖的体内免疫活性.以不同浓度的多糖作为药物组,环磷酰胺为阳性对照,生理盐水为阴性对照.给药14d后处死并解剖小鼠,眼眶取血,计算抑瘤率和免疫指数,检测血清中TNF-α、IL-2和IFN-γ含量.结果显示不同浓度的多糖表现出不同的免疫活性,其中多糖低浓度组(1.25mg/mL)有较高的肿瘤抑制率和免疫指数(P<0.01),并促进和调节IL-2、IFN-γ、TNF-α在机体内分泌(P<0.01),协调改善机体免疫系统功能,达到治疗肿瘤的目的.The in vivo immunomodulatory activities of the neutral polysaccahride extracted from Dendrobium denneanum were evaluated by using the transplantable models of S180 in mice.The different concentrations of neutral polysaccahride were given to the mice every day by intraperitoneal injection respectively,with CTX as the positive control and normal saline as negative control.These mice were anatomized after 14 d,and their blood samples were obtained from eye orbitae.Then,the tumor inhibition ratio,immune index and the concentration of cell factors（TNF-α,IL-2 and IFN-γ） were investigated,respectively.The results showed that the immunomodulatory activities were related to the concentration of the polysaccharide.At 1.25 mg/mL,the polysaccharide exhibited higher tumor inhibition ratio and immune index（P0.01）.DDP1-1（1.25 mg/mL） could improve the immunomodulatory effect of the mice by increasing the concentration of TNF-α,IL-2 and IFN-γ（P0.01） in serum.

关 键 词：迭鞘石斛 中性多糖 体内免疫 细胞因子 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]