免疫印迹技术在诊断新生儿先天梅毒中的意义  

DIAGNOSIS OF CONGENITAL SYPHILIS FOR NEONATES USING WESTERN BLOT

作　　者：郭玲[1] 赵梅桂[1] 李海[1] 邱勇龙[1] 张磊[1] 

机构地区：[1]深圳市宝安区慢性病防治院,深圳518133

出　　处：《现代预防医学》2010年第13期2438-2439,共2页Modern Preventive Medicine

摘　　要：[目的]探讨以血清为标本,通过免疫印迹技术诊断新生儿先天梅毒的临床意义。[方法]收集86例梅毒产妇所产新生儿血清,通过免疫印迹技术检测血清中梅毒特异性抗体,同时以梅毒螺旋体颗粒凝集试验为对照检测血清梅毒特异性抗体;对阳性新生儿进行18个月跟踪检测。[结果]免疫印迹检测显示梅毒产妇所产26例新生儿血清梅毒特异性抗体阳性,而通过梅毒螺旋体颗粒凝集试验呈现阳性者为23例;经过18个月跟踪检测,26例血清阳性的新生儿均被诊断为先天梅毒。[结论]免疫印迹技术诊断新生儿先天梅毒敏感性高于梅毒螺旋体颗粒凝集试验,该技术可以在临床推广。[Objective] Diagnosis of congenital syphilis for neonates was difficult without symptoms,this study aimed to explore the significance of diagnosis on neonates＇ congenital syphilis through serum samples using western blot.[Methods] Serum sample was collected from 86 neonates whose mothers with syphilis.Anti-Treponema pallidum antibodies were detected using western blot and Treponema pallidum particle agglutination test as control method.Neonates were followed up and tested for 18 months.[Results] Serum samples from 26 neonates whose mothers with syphilis were positive for anti-Treponema pallidum antibodies using western blot,23 neonates were found with positive anti-Treponema pallidum antibodies using particle agglutination test.All 26 neonates were diagnosed as congenital syphilis patients after 18 months observation and test.[Conclusion] Western blot is more sensitive for diagnosis of congenital syphilis for neonates than particle agglutination test.Western blot can be applied as diagnosis method of congenital syphilis for neonates.

关 键 词：免疫印迹 先天梅毒 梅毒螺旋体颗粒凝集试验 

分 类 号：R759.151[医药卫生—皮肤病学与性病学]