新型抗菌纳米复合HA-Gel骨修复材料的仿生合成及细胞相容性研究  

Biomimetic synthesis of a novel antibacterial nano-composite materials of hydroxyapatite and gelatin for bone repair and its biocompatibility in vitro

作　　者：窦晓晨[1] 李全利[1,2] 周健[1,2] 汤健[3] 

机构地区：[1]安徽医科大学附属口腔医院,安徽合肥230032 [2]安徽口腔疾病研究省级实验室,安徽合肥230032 [3]安徽医科大学第一附属医院,安徽合肥230032

出　　处：《上海口腔医学》2010年第3期285-289,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30772440);安徽省科技攻关项目(08010302196)

摘　　要：目的:制备一种新型的抗菌纳米复合骨修复材料,并评价其细胞相容性。方法:将一定量的羟基磷灰石(hydroxyapatite,HA)、米诺环素(minocycline,M)的盐酸溶液加入明胶(gelatin,Gel)水溶液中,调整反应体系的pH值至7～8,获得含有抗菌药物米诺环素的明胶-羟基磷灰石纳米复合水胶体。复合材料与骨髓基质干细胞(bone marrow stromal cells,BMSCs)共同培养,观察细胞的黏附、生长。结果:纳米级、低结晶度的HA均匀地分布于Gel中。复合材料具有类骨的微孔结构,可促进骨髓基质干细胞黏附、增殖、分化。结论:复合材料具有良好的细胞相容性,是一种颇有前途的骨组织修复材料。PURPOSE： To evaluate the bioactivity of a new type of antibacterial nano-composite materials of low- crystallinity hydroxyapatite and gelatin for bone repair in vitro. METHODS： The nanocomposite was prepared by a biomimetic method. A certain amount of acidic solution containing hydroxyapatite and minocycline was added into gelatin solution at 40℃ and pH 7-8 to obtain HA-Gel composite containing anti-bacterial drug minocycline. The material was co-cultured with rat bone marrow stromal cells（BMSCs） in vitro. RESULTS： The results indicated that the nanoscopic low-crystallinity hydroxyapatite was distributed evenly in the fibrils of the composite by controlling crystal growth. The HA -Gel nanocomposite had complicated porous structure. It could promote BMSCs adhesion, proliferation, and differentiation in vitro. CONCLUSIONS： The BMSCs response to the material considerably shows that it is bioactive. The nanocomposite may be promising as a kind of materials for bone replacement and a carrier for controlled -release therapeutic agents.Supported by National Natural Science Foundation of China（Grant No.30772440） and Key Science and Technology Research Project of Anhui Province（Grant No.08010302196）.

关 键 词：明胶 分子仿生 骨修复材料 抗菌 骨髓基质干细胞 

分 类 号：R318.08[医药卫生—生物医学工程]