三疣梭子蟹Profilin基因全长cDNA的克隆与表达分析  

cDNA Cloning, Characterization and mRNA Expression of a Profilin from the Swimming Crab Portunus trituberculatus

作　　者：申望[1] 叶茂[1] 石戈[1] 王日昕[1] 

机构地区：[1]浙江海洋学院海洋科学学院海洋生物资源及分子工程实验室,浙江舟山316004

出　　处：《Zoological Research》2010年第3期261-267,共7页动物学研究（英文）

基　　金：国家科技支撑计划(2007BAD43B08);浙江省科技厅重大项目(2007C02001);浙江省面上项目(2009C32019)

摘　　要：三疣梭子蟹(Portunus trituberculatus)是我国沿海重要养殖品种之一。近年来,养殖病害呈逐年上升趋势,制约了三疣梭子蟹养殖产业的健康可持续发展。克隆三疣梭子蟹免疫相关基因、研究免疫基因的功能和作用机制,可为三疣梭子蟹养殖病害的防治奠定基础。本研究从三疣梭子蟹血细胞全长cDNA文库中克隆了742bp的profilin基因全长cDNA。该profilin全长cDNA中开放阅读框长375bp,编码125aa。推导的三疣梭子蟹profilin理论等电点pI5.87,氨基酸序列与冈比亚按蚊(Anopheles gambiae)profilin同源性最高,序列一致性为42.9%。荧光定量RT-PCR分析结果显示,在正常的三疣梭子蟹机体中,血细胞profilin表达水平最高,其次为肝胰脏;在致病菌副溶血弧菌(Vibrio parahaemolyticus)诱导后,血细胞中profilin表达量显著上升(P<0.01),表明profilin可能参与了三疣梭子蟹的免疫防御反应,是一个免疫相关因子。We isolated and characterized the profilin full-length cDNA from hemocytes of swimming crab Portunus trituberculatus. The profilin cDNA consists of 742 bp and the 375 bp open reading frame encodes a polypeptide of 125 amino acids, having a predicted isoelectric point of 5.87. The deduced amino acid sequence shows 42.9% amino acid sequence identity to the profilin of mosquito Anopheles gambiae. The profilin mRNA was highly expressed in hemocytes and moderately in hepatopancreas of normal crab. The higher expression of profilin mRNA is observed in crab challenged by the pathogenic bacterium Vibrio parahaemolyticus. These results suggest a potential role for profilin in pathogen host defense mechanisms.

关 键 词：三疣梭子蟹 免疫 基因克隆 PROFILIN 基因表达 

分 类 号：Q78[生物学—分子生物学]