拟南芥SWR1复合体亚单位编码基因ARP6和SWC6的水稻同源基因功能研究  

Preliminary study on the function of rice orthologs of the two genes,ARP6 and SWC6 encoding the subunits of Arabidospsis SWR1 complex

作　　者：许永汉[1] 邓敏娟[1] 鲍烈明[1] 彭建斐[2] 胡张华[1] 

机构地区：[1]浙江省植物代谢基因工程重点实验室,浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021 [2]湖南农业大学生物安全科学技术学院,湖南长沙410128

出　　处：《浙江农业学报》2010年第3期269-275,共7页Acta Agriculturae Zhejiangensis

摘　　要：用RT-PCR的方法分别克隆出拟南芥SWR1复合体亚单位编码基因ARP6和SWC6在水稻中的同源基因OsARP6和OsSWC6,对其组织表达特异性进行了分析。构建这两个基因的表达载体,分别转化拟南芥突变体arp6和swc6,它们在阳性植株表达后均能在一定程度上使突变性状回复,表明在拟南芥和水稻之间,这些基因在功能上具有一定的保守性。本研究结果为水稻中存在SWR1功能复合体提供了重要的证据,也有助于探明该复合体调控基因表达的分子机制。In this study,OsARP6 and OsSWC6 in rice,the orthologs of the two genes ARP6 and SWC6 encoding the subunits of Arabidopsis SWR1 complex,respectively,were cloned via RT-PCR and their expression pattern was analyzed.We constructed their expression vectors and then used the vectors to transform Arabidopsis mutants arp6 and swc6,respectively.In the transformants,mutated traits had been rescued by the ectopic expression of OsARP6 or OsSWC6,demonstrating the high conversation of these homologue in function and construction existed between the rice and Arabidopsis.These results provided valuable evidence that there would exist the SWR1 complex in rice,which was of importance for unveiling its molecular mechanism of regulating gene expression.

关 键 词：水稻 SWR1复合体 亚单位 拟南芥 互补 

分 类 号：Q78[生物学—分子生物学]