逐痰通络汤对局灶性脑缺血大鼠凝血纤溶系统的影响  

Effect of Zhutan Tongluo Tang on the coagulation and fibrinolysis of the focal cerebral ischemia in rats

作　　者：周坚[1] 刘时觉[1] 王小同[2] 陈醒言[3] 郑国庆[2] 

机构地区：[1]温州医学院附属第二医院中医科,浙江温州325027 [2]温州医学院附属第二医院神经内科,浙江温州325027 [3]温州医学院药学院

出　　处：《温州医学院学报》2010年第3期256-258,共3页Journal of Wenzhou Medical College

基　　金：浙江省中医药科研基金资助项目(2004C129);温州市重大科研基金资助项目(Y20070038w)

摘　　要：目的:观察逐痰通络汤对局灶性脑缺血大鼠凝血纤溶系统的影响。方法:清洁级雄性SD大鼠18只,随机分为假手术组(K组)、模型组(C组)和逐痰通络汤组(Z组),每组各6只。应用线栓法建立大脑中动脉栓塞(MCAO)模型。造模后第7天观察各组大鼠神经功能缺损并评分,脑组织TTC染色并测定梗死体积,以发色底物法测定血浆组织型纤溶酶原激活物(t-PA)、组织型纤溶酶原激活抑制物(PAI)、纤溶酶原(PLG)活性,ELISA法测定血浆D-二聚体(DD)活性。结果:C组、Z组均出现不同程度神经功能缺损和梗死灶。与C组相比,Z组神经功能缺损较轻、梗死体积较小(P<0.05)。C组、Z组PLG、PAI、DD活性显著高于K组,t-PA活性显著低于K组(P<0.01)。Z组PLG、PAI、DD活性低于C组(P<0.05,P<0.01),t-PA活性高于C组(P<0.05)。结论:增强血浆纤溶活性,纠正凝血纤溶系统失衡,可能是逐痰通络汤治疗脑缺血的机制。Objective：To observe the effect of Zhutan Tongluo Tang on the coagulation and fibrinolysis of focal cerebral ischemia in rats.Methods：Eighteen Sprague-Dawley male rats were divided randomly into 3 groups：sham operation group（K）,model group（C） and Zhutan Tongluo Tang group（Z）.The middle cerebral artery occlusion（MCAO） model was established with the thread-tying method.At 7 days after operationt,he scores of neural functional defect were measured,the tissues of brain were stained with TTC to measure infarct area,the activity of t-PA,PAI and PLG was measured using CLIA.The activity of DD was measured using ELISA.Results：Rats of C and D group had neural functional defect and infarct area with different degree.Compared with C group,the neural functional defect of Z group was lighter and the infarct area was smaller（P 0.05）.The activity of PLG,PAI and DD in C and D groups was significant higher than that of K group but t-PA was significant lower（P 0.01）T.he activity of PLG,PAI and DD in Z group was lower than that of C group（P 0.05,P 0.01）but t-PA was higher（P 0.05）.Conclusion：Promot-ing activity of fibrinolysis system and correcting disequilibrium of coagulation and fibrinolysis system are probably mechanism that Zhutan Tongluo Tang treats ischemia.

关 键 词：逐痰通络汤 脑缺血 凝血纤溶系统 大鼠 

分 类 号：R2[医药卫生—中医学]