卵泡抑素参与爪蟾心的形成  

Follistatin is required for heart formation in xenopus laevis

作　　者：赵勇[1] 周影[2] 秦书俭[3] 高东明[4] 

机构地区：[1]辽宁医学院生物化学教研室,锦州121001 [2]辽宁医学院附属第一医院,锦州121001 [3]辽宁医学院解剖学教研室,锦州121001 [4]辽宁医学院生理学教研室,锦州121001

出　　处：《解剖学杂志》2010年第3期307-309,327,共4页Chinese Journal of Anatomy

摘　　要：目的：研究卵泡抑素在爪蟾心形成过程中的作用。方法：切下发育第14期胚胎形成心的组织，并分别加入卵泡抑素、骨形成蛋白和活化素进行培养。4h时后，用MEMPHA固定并进行胚胎整体原位杂交。利用Morpholio Oligo技术减弱了卵泡抑素的表达，检测了在缺乏卵泡抑素条件下心形成的变化。结果：早期心表达卵泡抑素；增加或降低卵泡抑素均抑制Nkx2．5的表达。结论：卵泡抑素在心的形成过程中发挥十分重要的作用，缺乏或过量卵泡抑素均影响心的形成。Objective： To investigate the role of follistatin in the heart formation in xenopus laevis. Methods： Explants from embryos of xenopus laevis at stage 14 were isolated for culture with follistatin, BMP and activin. Four hours later, RNA tran- scripts were examined by whole mount in situ hybridization. For morpholino experiments, embryos were injected at one-ceil stage with 5 or 10 ng of foltistatin-MO or control MO solution. At stage 20, RNA transcripts were examined by whole mount in situ hybridization. Results： Follistatin was expressed in the early heart as well as in the neural tissues; Either overexpression of follistatin or deficient follistatin inhibited Nkx 2.5 expression in the heart explants. Conclusion： Follistatin is a key player in heart development and plays an essential role in regulating heart formation in xenopus laveis.

关 键 词：卵泡抑素 心 形成 爪蟾 胚胎 

分 类 号：R339.22[医药卫生—人体生理学]