猪囊尾蚴头节单克隆抗体的制备及特性鉴定  

Preparation and characterization of the monoclonal antibodies against Cysticercus Cellulosae scolex

作　　者：蔡亚南[1] 张晓雷 王泽[3] 徐鹏[4] 杨桂连[1] 刘春杰[1] 姜晶[1] 赵权[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]长春市动物检疫站,吉林长春130061 [3]吉林大学畜牧兽医学院,吉林长春130062 [4]锦州医学院畜牧兽医学院,辽宁锦州121001

出　　处：《中国预防兽医学报》2010年第6期485-487,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金资助项目(30571396)

摘　　要：为制备抗猪囊尾蚴头节单克隆抗体(MAb),本研究采用纯化的猪囊尾蚴头节(Cysticercus Cellulosae Scolex)作为抗原免疫BALB/c小鼠,通过杂交瘤细胞技术,获得3株分泌抗猪囊尾蚴头节MAb的杂交瘤细胞系(分别命名为2C6、3A5和4G10)。对这3株MAb进行生物学特性鉴定,其染色体平均记数为92±10对,经间接ELISA检测腹水效价分别为1:12800、1:6400和1:25600。亚类鉴定证实,这3株亚类均为IgG1型。Westernblot检测表明,3株纯化的MAb均可与猪囊尾蚴头节抗原发生特异反应;而与猪囊尾蚴囊壁和囊液抗原均不发生反应。本研究获得的3株MAb为猪囊尾蚴病免疫学诊断研究奠定技术基础。Three hybridoma cell lines secreting MAb （designated as 2C6, 3A5 and 4G10） against Cysticercus Cellulosae scolex antigen were developed by hybridoma techniques. These hybridoma had 92 ± 10 pairs of chromosomes and all three MAbs belonged to IgG1 subclass. The ascites antibody titer of the MAb were 1：12 800, 1：6 400 and 1：25 600 repectively. Western blot showed that these MAbs could specifically recognize certain C.cellulosae scolex antigen, but not cysticercosis cyst fluid antigen and capsule wall antigen.

关 键 词：猪囊尾蚴头节 单克隆抗体 制备 鉴定 

分 类 号：S852.7[农业科学—基础兽医学]