番茄E8基因启动子的克隆及其在拟南芥中的表达  

Cloning E8 Promoter from Tomato and Expression of E8 Gene in Transgenic A. thaliana

作　　者：张艳云[1] 林卫[1] 李唯[1] 马静芳[1] 王旺田[1] 

机构地区：[1]甘肃农业大学生命科学与技术学院,兰州730070

出　　处：《生物技术通报》2010年第7期113-116,124,共5页Biotechnology Bulletin

基　　金：甘肃省科技厅计划项目(0708NKCA070)

摘　　要：利用PCR技术从番茄基因组DNA中克隆长约1.1kb的E8基因启动子与517bp的E8基因片段,将其二者连接构建植物表达载体,导入农杆菌,通过花序侵染法转化拟南芥,得到转基因拟南芥。用RT-PCR分析转基因拟南芥中E8基因启动子驱动E8基因小片段的结果表明,E8基因小片段mRNA仅在转基因拟南芥长角果中转录,根、茎、叶、花中不转录,提示E8基因的1.1kb启动子在异源植物拟南芥中仍具有驱动外源基因果实特异性表达的特性。The 1 100 bp 5＇ flanking sequence and 517 bp partial encode sequence of E8 gene was cloned by PCR. The promoter was fused with small fragement of E8 gene to construct plant expression vector pCAMBIA2300-E8 ,which was introduced into A. thaliana by Agrobacterium-tumefaciens. RT-PCR results which analysis of E8 1. 1 promoter driving E8 gene in transgenic A. thaliana showed that E8 partial encode gene only expressed in the siliques, but not in root,leaf,stem,flower. The above results suggest that E8 promoter also has ability of driving exogenous gene expressed＇in transgenic A. thaliana. This result can make possihle to use E8 promoter in transgenic peach on subsequent research.

关 键 词：E8启动子 拟南芥 E8基因小片段 基因表达 

分 类 号：Q943.2[生物学—植物学]