ELISA检测人降钙素基因相关肽在转基因马铃薯块茎中的专一性表达  

Tuber-specific Expression of Human Calcitonin-gene Related Peptide in Transgenic Potato by ELISA

作　　者：宋东光[1] 邓日烈[1] 聂呈荣[1] 张英慧[1] 陈淑珍[1] 仇月明[1] 汪训明[2] 

机构地区：[1]佛山科学技术学院园艺系,佛山528231 [2]复旦大学遗传所,上海200433

出　　处：《生物技术通报》2010年第7期117-119,共3页Biotechnology Bulletin

基　　金：广东省自然科学基金资助项目(000014)

摘　　要：通过间接ELISA检测了人CGRP基因在转基因马铃薯中的表达。马铃薯class I patatin基因5'侧翼区驱动下人CGRP的表达表现了明显的块茎专一性,茎段中没有检测到CGRP活性,CGRP的表达量在不同转基因株系中有差异,最高达到约50ng/mg可溶性蛋白。Human calcitonin-gene related peptide（CGRP） expression was detected in transgenic potato by indirect ELISA in this study. The human CGRP expression pattern was tuber-specific directed by 5 ＇flanking region of potato class I patatin gene, but no aetivity was detected in stems. Differences were observed in trausgenic lines with highest of about 50 ng/mg soluble protein. This work lays foundation for neuronal pcptide production by transgenic plant bioreactors.

关 键 词：人CGRP ELISA 转基因马铃薯 

分 类 号：S532[农业科学—作物学]