冬虫夏草培养子实体ITS,5.8S的分析及系统发育研究  

ITS and 5. 8S Analysis and Phylogenetic Relationship of Culture Fruiting Body of Cordyceps sinensis( Berk. ) Sacc.

作　　者：彭慧超[1,2] 程大志 王爱珍[4] 王环[1] 王海庆[1] 沈建伟[1,2] 司庆文[1,2] 韩发[1] 周党卫[1] 

机构地区：[1]中国科学院西北高原生物研究所高原生物进化与适应开放实验室,西宁810001 [2]中国科学院研究生院,北京100049 [3]青海世峰生物科技有限公司,西宁810003 [4]青海大学生命科学系,西宁810016

出　　处：《生物技术通报》2010年第7期128-133,141,共7页Biotechnology Bulletin

基　　金：中科院知识创新项目(KSCX2-SW-106);青海省科技厅星火计划项目;中科院西北高原生物研究所知识创新前沿项目

摘　　要：对野生冬虫夏草的人工培养子实体的ITS1,ITS2和5.8S间区序列进行了克隆,并结合已有的序列对ITS1,ITS2和5.8S进行了系统发育分析。结果表明,人工培养子实体与中华被毛孢(Hirsutella sinensis L95DBM-1)具有96%的同源性,在NJ邻接树上形成明显的一支,与虫草属其它类群种间具有明显差异,表明分离培养的虫草子实体为中华被毛孢。遗传距离分析结果显示,培养虫草序列与已知虫草在种内也存在一定差异,可能暗示虫草种群在不同区域具有一定的遗传分化。The ITS-5.8S sequences were cloned on the culture fruiting body of Cordyceps sinensis. Based on some known ITS- 5.8S sequences in Cordyceps genus,the molecular phylogenetics relationship was performed. The results showed that the ITS-5.8S sequence of the culture asexual fruiting body had 96% identity to that of Hirsutella sinensis（ L95DBM-1 ） and clustered on the obvious lineage. The sequence of culture asexual was different from that of the other species of Cordyceps genus in ITS-5.8S Neighbor-Join phylogenetics tree. This showed that the culture asexual fruiting body was the Hirsutella sinensis. Divergence distance analysis showed that the ITS-5.8S sequence of the culture asexual fruiting body also had great difference in inter-species of C. sinensis,which may suggest that C. sinensis population have certain genetic divergence in different region.

关 键 词：虫草 ITS.5.8S 系统发育分析 中华被毛孢 

分 类 号：S567.35[农业科学—中草药栽培]