微小隐孢子虫p23基因在干酪乳杆菌中的表达及其表达产物的鉴定  

The surface antigen p23 gene from Cryptosporidium parvum sporozoites expressed in Lactobacillus casei zhang and identification of its expression products

作　　者：格日勒图[1] 布仁贺希格[2] 白梅[1] 贾洪林 玄学南 张和平[1] 

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,内蒙古呼和浩特010018 [2]内蒙古自治区农业综合开发评审中心,内蒙古呼和浩特010098 [3]日本带广畜产大学国家原虫病研究中心遗传生化研究分野

出　　处：《中国兽医学报》2010年第6期770-773,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30960279);内蒙古农业大学"中国博士后流动站"项目(57545);内蒙古农业大学"博士启动基金"项目(BJ05-02)

摘　　要：应用DNA重组技术把微小隐孢子虫(Cryptosporidium parvum)子孢子抗原p23基因克隆入表达载体pMG36e当中,成功构建了可在干酪乳杆菌(Lactobacillus casei Zhang)胞浆中表达的重组载体pMG-p23。并以SDS-PAGE、Western blotting以及IFAT试验方法,分别在破碎菌体组分和活菌体当中鉴定p23基因表达产物并确定其在细胞中表达的位置。本试验为进一步构建分泌型或锚定型干酪乳杆菌(L.casei Zhang)活菌载体奠定了基础。A surface antigen p23 gene of Cryptosporidium parvum sporozoites was successfully cloned into expression vector pMG36e,and the recombinant vector pMG-p23 was transformed into Lactobacillus casei Zhang.The p23 gene was stably expressed in cytoplasm form L.casei Zhang and its expression products was detected by SDS-PAGE,Western blotting and the IFAT testing method and located in cytoplasm.The results indicate that the pMG-p23 derived from pMG36e can act as a L.casei cytoplasm expression vector,and the research established a basis on constructing recombinant vectors of secretion and anchor type in the live bacteria.

关 键 词：干酪乳杆菌 微小隐孢子虫 p23基因 表达 

分 类 号：S852.72[农业科学—基础兽医学] S852.61[农业科学—兽医学]