镉诱导仔猪睾丸支持细胞氧化酶活性降低及DNA的损伤  

DNA damage and decrease of cellular oxidase activity in piglet sertoli cells exposed to cadmium

作　　者：张明[1,2] 邬静[1] 袁慧[1] 邓思君[1] 袁莉芸[1] 郭成志[1] 朱丽[1] 

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128 [2]江西生物科技职业学院,江西南昌330200

出　　处：《中国兽医学报》2010年第6期818-821,共4页Chinese Journal of Veterinary Science

基　　金：湖南农业大学青年基金(07QN06);湖南农业大学动物医学院青年基金(BK0718)

摘　　要：以仔猪睾丸支持细胞为试验模型,采用二步酶消化法分离支持细胞进行培养。探讨了0、10、20、40、80μmol/L的氯化镉对支持细胞的毒性作用。结果表明:10μmol/L以上的氯化镉有抑制支持细胞生长的作用,并能使支持细胞氧化酶活性下降,造成支持细胞DNA的损伤。The study was designated to explore the toxic effects of cadmium on piglet sertoli cells. Sertoli cells were isolated from piglet testes uisng a two-step enzyme digestion and followed by differential plating. Piglet sertoli cells were cultured and classified into five groups, i.e, group A, control without cadmium chloride; group B with 10 μmol/L cadmium chloride; group C with 20 μmol/L cadmium chloride; group D with 40 μmol/L cadmium chloride; and group E with 80 μmol/L cadmium chloride. We found that sertoli cells＇ growth was inhibited by arsanilic acid at 10 μmol/L compared with the control group A. The oxidase activity of sertoli cells was decreased by arsanilic acid at 10 μmol/L. Moreover, DNA damage of sertoli cells was observed by arsanilic acid at 10 μmol/L.

关 键 词：氯化镉 支持细胞 氧化酶 DNA损伤 

分 类 号：S852.2[农业科学—基础兽医学] S884.9[农业科学—兽医学]