牛孤雌激活囊胚冷冻复苏后胚胎存活率和胚胎质量  

The survival rate and embryonic quality of bovine parthenogenetic blastocysts post-vitrification cryopreservation

作　　者：张鹏[1] 王中伟[1] 马馨[2] 高飞[1] 唐博[1] 张嘉保[1] 李子义[1] 

机构地区：[1]吉林大学畜牧兽医学院动物胚胎工程吉林省重点实验室,吉林长春130062 [2]吉林农业大学动物科技学院,吉林长春130118

出　　处：《中国兽医学报》2010年第6期847-851,共5页Chinese Journal of Veterinary Science

基　　金：国家科技支撑计划资助项目(2007BAD55B03);国家转基因生物新品种培育重大专项(2009ZX08007-004B)

摘　　要：使用2种不同的冷冻载体冷冻保存牛孤雌囊胚,并且使用差异染色的方法计数囊胚细胞数。结果显示,开放式毛细玻璃管(GMP)和冻精管(Straws)冷冻牛孤雌囊胚复苏后囊胚孵出率存在显著性差异(GMP法为70.29%,冻精管法为27.31%,P<0.05)。差异染色结果显示,牛囊胚冷冻复苏后囊胚细胞数目为91.37,而对照组(正常孤雌囊胚)囊胚细胞数目为93.70,两者差异不显著(P>0.05),并且前者内细胞团数(ICM)与滋养层细胞数(TE)比例为0.23,而后者为0.24,差异不显著(P>0.05)。结果表明,GMP法更适合冷冻保存牛孤雌囊胚,并且其冷冻复苏后囊胚的孵出率达到70.29%。同时使用GMP冷冻囊胚对囊胚细胞的损伤较小。Cryopreservation of oocytes and embryos is a crucial step for conservation of animal genetic resources,and vitrification is becoming a very useful method for embryo cryopreservation.In this study,two types of vectors （glass micropipette （GMP） and straws） and a differential staining method were used to investigate the survival rate and embryonic quality of bovine parthenogenetic blastocysts post-vitrification cryopreservation.Our results showed the hatching rate （70.29%） of blastocysts loaded into GMP was significantly higher （P0.05） than that of （27.31%） loaded into straws post-vitrification.By the differential staining,the total cells of non-vitrified parthenogenetic bovine blastocysts was 93.70,which was higher （P0.05） than that （91.37） of vitrified blastocysts.And,no significant difference （P0.05） was seen on ratios between vitrified blastocysts （ICM/TE=0.24） and non-vitrified blastocysts （ICM/TE=0.23）.Our results demonstrate that a glass micropipette vector was much better than a straw in vitrification cryopreservation of bovine parthenogenetic blastocysts,which caused less damage on blastocyst cells.This study lays the foundation for improving a method of vitrification cryopreservation of bovine embryos and the differences of embryos cryosensitivity and cells counts between normal embryos and freezable embryos.

关 键 词：孤雌激活 囊胚 差异染色 玻璃化冷冻 

分 类 号：S823[农业科学—畜牧学] S814[农业科学—畜牧兽医]