中国西门塔尔牛、利木赞牛和夏洛莱牛MSTN基因多态性分析  

Analysis of Chinese Simmental,Limousin and Charolais of MSTN gene polymorphism

作　　者：吕文发[1] 杨连玉[1] 贺实伟[1] 杨雨江[1] 张嘉保[2] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118 [2]吉林大学实验动物中心,吉林长春130062

出　　处：《中国兽医学报》2010年第6期852-854,868,共4页Chinese Journal of Veterinary Science

基　　金：国家科技支撑计划资助项目(2007BAD55B03-03);国家自然科学基金资助项目(30972108)

摘　　要：分别以20头中国西门塔尔牛、利木赞牛和夏洛莱牛为研究对象,采用创造酶切位点PCR与降落PCR相结合的方法对3个品种的基因外显子1的突变位点扩增,分析了3个肉牛品种之间的Myostain(MSTN)基因多态性。运用1对特异性引物扩增3个肉牛品种的MSTN基因已知外显1SNP位点,扩增片段大小为217bp。运用CRS-RFLP方法鉴定60头牛MSTN基因在3个群体中的分布情况。结果表明,在西门塔尔群体中等位基因C占优势,等位基因频率为60%,AA基因型频率为0;在夏洛莱群体中等位基因A优势,等位基因频率为57.5%,CC基因型频率为0;而在利木赞群体中未检测到这一突变。Analysis of MSTN （Myostain） gene polymorphism were studied by descend PCR and CRS-PCR in 20 Chinese Simmental,Limousin and Charolais each,respectively. The known exon 1 SNP of MSTN gene was performed by using a pair of primer.Distribution of MSTN gene among the 60 cattles in three breeds was tested through CRS-RFLP method.The results showed that C allegene was advantage in Chinese Simmental,gene frecquce was 60%,AA genotype francequce was 0;A allegene was advantage in Charolaise cattle,gene frecquce was 57.5%,CC genotype francequce was 0;MSTN mutation were not detected in Limousin cattle.

关 键 词：MSTN 基因多态性 创造酶切位点PCR 降落PCR 肉牛 

分 类 号：S823[农业科学—畜牧学] S852.2[农业科学—畜牧兽医]