蛇毒神经生长因子生物活性研究  

The study of biological Activity of nerve growth factor from snake venom

作　　者：王立兰[1] 田霖[1] 乐嘉静[1] 吕慧贤[1] 马霄[1] 骆鹏[1] 

机构地区：[1]中国药品生物制品检定所

出　　处：《微生物学免疫学进展》1999年第1期51-51,共1页Progress In Microbiology and Immunology

摘　　要：从蛇毒中分离的神经生长因子（ＮＧＦ），经用鸡胚背根神经节做生物活性测定，发现能促进神经节树突最大生长的最小ＮＧＦ浓度为２．５ｎｇ／ｍｌ。半成品的毫克蛋白（ｍｇＰ）比活性在１．５４×１０５～５．３５×１０５Ｕ／ｍｇＰ之间。经用ＨＰＬＣ和ＳＤＳ－ＰＡＧＥ电泳做纯度分析，主峰面积在９５％以上。Nerve Growth Factor(NGF) was purified from snake crude venom.The biological activity was measured by the culturing the dorsal root ganglia of chicken embryo.It is found that it could promote the maximum neruite outgrowth in the miximum protein concentration 2.5ng/ml.The specific activity were between 1.54～5.35×10 5 U/mg protein.The purity of NGF was assayed by HPLC and SDS PAGE.Main peak was over 95% of the total area.We also screened a new excipient prescription for NGF products.It could maintain the good activity and have no side effects.These studies are going to promote the scientific research and production of NGF.

关 键 词：NGF 生物活性 比活性 纯度 

分 类 号：TQ464.9[化学工程—制药化工]