人骨髓CD34^+造血细胞体外扩增形成HPP-CFC造血祖细胞的能力  

EXPANSIVE AND GENERATIVE CAPACITIES OF HIGH PROLIFERATIVE POTENTIAL COLONY FORMING CELLS DERIVED FROM CD34 + HEMATOPOIETIC CELLS OF HUMAN BONE MARROW

作　　者：奚永志[1,2,3] 张双喜[1,2,3] 孔繁华[1,2,3] 金荔 陈兴国[1,2,3] 魏文 张志欣[1,2,3] 唐佩弦 

机构地区：[1]军事医学科学院附属医院免疫学研究室 [2]军事医学科学院基础医学研究所 [3]北京市中心血站白细胞研究室

出　　处：《中国应用生理学杂志》1999年第1期66-69,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金

摘　　要：目的和方法：高增殖潜能集落形成细胞（ＨＰＰＣＦＣ）是表达ＣＤ３４＋ＤＲＬｉｎ－的最早期造血祖细胞之一，它在体外的增殖分化能力可反映造血干细胞的某些特征。结果：本文研究了人正常骨髓ＣＤ３４＋造血细胞在体外扩增和形成ＨＰＰＣＦＣ的能力。利用ＣＩＭＳ１００免疫磁性分离术首先获得＞９０％的ＣＤ３４＋造血细胞以富集ＨＰＰＣＦＣ。在含有Ｅｐｏ＋ＧＭＣＳＦ＋ＩＬ３＋ＩＬ６＋ＳＣＦ（简ＥＧＩＩＳ）的无基质液培条件下，ＣＤ３４＋造血细胞在四周内可持续产生单个核细胞和ＨＰＰＣＦＣ，并使其总量最高可达１７７０倍和８倍，以第２和３周为最佳时期，但不同个体ＣＤ３４＋造血细胞的这种能力差别较大。结论：高度纯化的人骨髓ＣＤ３４＋造血细胞能够在含有最佳组合造血生长因子的无基质液培条件下持续扩增，为临床应用提供了重要依据。Aim and Methods: High proliferative potential colony forming cells (HPP CFC) as one of the most primitive hematopoietic progenitors with CD34 + DR - Lin - antigens have been identified and used as effective tools for examination of functional properties of hematopoietic stem cells. In this article, we investigated the in vitro generative and expansive potentials of both mononuclear cells and HPP CFC from CD34 + hematopoietic cells of human bone marrow.Results:Highly purified CD34 + hematopoietic cells obtained by the means of CIMS 100 immunomagnetic isolation system were maintained in stromal cells free liquid culture system (SCFLCS) with the supplement of the most potent combinations of EPO+GM CSF+IL3+IL6+SCF every 48 hours. At weekly intervals, the cells cultured in SCFLCS were demidepopulated, counted and assayed for HPP CFC. Within the alloted time of 4 weeks there were a marked increase in numbers of both mononuclear cells and HPF CFC respectively, albeit to a different magnitude and at different rates among different individuals. The most dramatic cellular and HPP CFC expansions were observed at 2nd and 3rd weeks.Conclusion: These data demonstrated that CD34 + hematopoietic cells could be significantly augmented ex vivo to generate large amount of primitive hematopoietic progenitor and mature cells.

关 键 词：造血细胞 CD34^+造血细胞 HPP-CFC 骨髓 

分 类 号：R331.22[医药卫生—人体生理学]