利多卡因对人中性粒细胞释放氧自由基的影响  

THE EFFECT OF LIDOCAINE ON OXYGEN FREE RADICAL PRODUCTION BY HUMAN NEUTROPHILS

作　　者：李永荣[1] 姚德厚[1] 郭贵林[1] 郁振帮 张信超[1] 

机构地区：[1]成都军区总医院麻醉科

出　　处：《中国应用生理学杂志》1999年第1期70-71,共2页Chinese Journal of Applied Physiology

摘　　要：目的：研究利多卡因对人中性粒细胞释放氧自由基的影响。方法：分别用５、１０、２０、５０μｇ／ｍｌ利多卡因与中性粒细胞孵育３０ｍｉｎ（３７℃），再用趋化寡肽（ｆｍＬＰ）或佛波酯（ＰＭＡ）刺激，用化学发光法测定ｆｍＬＰ或ＰＭＡ介导的中性粒细胞化学发光反应。结果：上述各浓度的利多卡因对ｆｍＬＰ介导的中性粒细胞化学发光反应有显著的抑制作用，其抑制率分别为４７２２％、７６６３％、８４４３％、８６７２％。当用ＰＭＡ刺激时，利多卡因显著抑制其介导的中性粒细胞化学发光反应。Aim:To investigate effect of lidocaine on oxygen free radical production by human neutrophils. Methods:neutrophils were preincubated with lidocaine(5,10,20,50μg/ml) at 37℃ for 30 minutes, Chemiluminescence in neutrophils induced by fmLP or PMA were examined by chemiluminescence assay. Results:Various concentrations of lidocaine inhibited chemiluminescence of neutrophils induced by fmLP significantly,and inhibitory ratio were 47.22%, 76.63%,84.43%,86.72% respectively.Alternatively,PMA, as a neutrophils stimulator,was also used in this assay system and similar results were observed. Conclusion: lidocaine inhibits oxygen free radical production by human neutrophils.

关 键 词：中性粒细胞 氧自由基 利多卡因 

分 类 号：R329.27[医药卫生—人体解剖和组织胚胎学] R971.2[医药卫生—基础医学]