骨髓基质干细胞诱导分化为神经元过程中miR-124和miR-128的表达  

EXPRESSIONS OF miR-124 AND miR-128 IN THE PROCESS OF BONE MARROW STROMAL CELLS DIFFERENTIATING INTO NEURAL CELLS

作　　者：王晶晶[1] 王春芳[1] 党雯[2] 李鹏飞[1] 蔚洪恩[1,3] 

机构地区：[1]山西医科大学生物技术教研室,太原030001 [2]山西医科大学附属第二医院药剂科,太原030001 [3]第二军医大学解剖教研室,上海200433

出　　处：《中国组织化学与细胞化学杂志》2010年第3期221-224,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金(30672114)

摘　　要：目的探讨骨髓基质干细胞诱导分化为神经元过程中miR-124和miR-128的表达变化及作用。方法采用全骨髓培养法体外分离培养获得骨髓基质干细胞,取传代培养至第3代的骨髓基质干细胞,在神经干细胞培养液及细胞因子等条件下诱导其分化为神经元,倒置显微镜下观察其形态变化,应用ABI公司的TaqManMicroRNAAssaysreal-timePCR技术,检测miR-124和miR-128在诱导分化过程中的表达。结果 miR-124分化后神经元的表达是未分化BMSCs的0.051倍(P<0.05);miR-128分化后神经元的表达是未分化BMSCs的0.070倍(P<0.05)。结论 miR-124和miR-128在骨髓基质干细胞诱导分化为神经元过程中可能起重要作用。Objective To detect the changes and effects of miR-124 and miR-128 in the process of bone marrow stromal cells （BMSCs） differentiating into neural cells.Methods BMSCs were isolated and cultured in vitro. Then BMSCs of passage 3 were induced to differentiate into neural cells. The morphologic features and growth status of BMSCs and differentiated BMSCs were observed by inverted microscopy. ABI＇s TaqMan MicroRNA Assays real-time PCR was used to measure the expression of miR-124 and miR-128 in the BMSCs before and after induction.Results The level of miR-124 in differentiated BMSCs was 0.051-fold of that in BMSCs （P0.05）,and the level of miR-128 in differentiated BMSCs was 0.070-fold of that in BMSCs （P0.05）.Conclusion Both miR-124 and miR-128 may play an important role in the process of bone marrow stromal cells differentiating into neurons.

关 键 词：骨髓基质干细胞 分化 神经元 miR-124 miR-128 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]