大鼠局灶性脑缺血后缺血边缘区NG_2细胞的表达  

EXPRESSION OF NG_2 CELLS IN ISCHEMIA BOUNDARY ZONE AFTER MCAO IN RATS

作　　者：高杰[1,2] 张桂青[1] 喻志源[2] 王伟[2] 谢敏杰[2] 

机构地区：[1]新疆石河子大学医学院护理系,石河子832008 [2]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2010年第3期225-228,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金(30971007)

摘　　要：目的观察局灶性脑缺血后缺血边缘区海马和皮层NG2细胞的动态表达,探讨其在脑缺血神经损伤与修复过程中所起的作用。方法将大鼠随机分为假手术组(sham)和脑缺血再灌注组,脑缺血再灌注组采用线栓法制备大鼠大脑中动脉阻塞再灌流模型(MCAO),假手术组不插入线栓,采用免疫荧光组织化学法结合共聚焦显微镜成像观察sham组及脑缺血后3d,7d,30d不同时间点缺血边缘区的海马CA1区和皮层区NG2的动态表达情况。结果脑缺血再灌注后缺血边缘区海马和皮层NG2胶质细胞表达增加,缺血后7d最明显。结论脑缺血后缺血边缘区存在NG2细胞的增生和形态变化可能与脑缺血后损伤修复密切相关。Objective To observe the expression of NG2 in ipsilateral hippocampus and cortex of rats after middle cerebral artery occlusion （MCAO）.Methods Rats were randomly divided into the sham-operated group and ischemia group.Focal cerebral ischemia in rats was induced by occluding the middle cerebral artery for 1 hour followed by repufusion.Immunofluorescent staining was used to show the expression o fNG2.Results MCAO induced the increases of NG2-positive cells in ipsilateral hippocampus and cortex, which peaked at day 7 after ischemia.Conclusion Proliferation of NG2 cells may play an active role in the process of neuronal damage and repair after focal ischemia.

关 键 词：脑缺血 缺血边缘区 NG2细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]