小麦耐盐系愈伤组织细胞的超微结构观察  

THE ULTRASTRUCTURAL OBSERVATION OF WHEAT SALT TOLERANCE CELL LINES OF CALLI

作　　者：郑文菊[1] 王勋陵[1] 贾敬芬[2] 

机构地区：[1]兰州大学生物系 [2]西北大学生物系

出　　处：《西北植物学报》1999年第1期81-84,共4页Acta Botanica Boreali-Occidentalia Sinica

摘　　要：对用正筛选法选出的耐１％、２％和２．５％ＮａＣｌ的小麦３个耐盐细胞系进行了详细的电镜观察。结果表明，随着耐盐程度的提高，小麦愈伤组织细胞的质体呈膨胀状态，数量有减少的趋势。耐低盐浓度（１％ＮａＣｌ）的细胞系线粒体结构与对照差别不大，而耐高浓度盐分的细胞系与对照有明显差异。耐盐系细胞的线粒体、粗糙型内质网及核糖体数量比对照细胞增加，高尔基体和内质网膨胀，细胞核核质凝聚，核膜膨胀，液泡发达。The ultrastructures of three salt tolerant wheat cell lines of 1%、2% and 2.5% NaCl tolerance were selected by in vitro method,were observed by transmission electron microscopy in detail.The result showed that the plastids callus cells swelled,and the plastid number seemed to be decrease,with the increase of salt tolerant intensity.The mitochondria had not any bigger difference than control in low NaCl tolerance (1% NaCl).The numbers in mitochondria,rough endoplasmic reticulum and ribosomes of salt tolerant cells increased by control,Golgi body and ER swelled,cytoplasm agglomerated,nuclei membrane swelled,vacuoles developed.Any ultrastructural changes in cell walls were not observed.

关 键 词：氯化钠 小麦 耐盐系愈伤组织 超微结构 

分 类 号：S512.101[农业科学—作物学] Q949.714.2[生物学—植物学]