TF-1细胞凋亡相关基因的研究  

Studies on the Apoptosis Related Genes of TF 1 Cell Line by cDNA RDA Technique.

作　　者：刘红涛[1] 王玉刚[1] 张颖妹[1] 宋泉声[1] 敬保迁 袁勇[1] 马大龙[1] 

机构地区：[1]北京医科大学卫生部医学免疫学重点实验室

出　　处：《生物化学与生物物理进展》1999年第1期38-43,共6页Progress In Biochemistry and Biophysics

摘　　要：利用近年来发展起来的代表差异分析（ｃＤＮＡｒｅｐｒｅｓｅｎｔａｔｉｏｎａｌｄｉｆｅｒｅｎｃｅｓａｎａｌｙｓｉｓ，ｃＤＮＡＲＤＡ）技术研究了在人红白血病细胞株ＴＦ１细胞撤除细胞因子后进入凋亡时诱导表达的基因．发现了６个新基因片段．其中有三个经与ＧｅｎＢａｎｋｎｒ和ｄｂＥＳＴ查询均没有发现同源性，已经向ＧｅｎＢａｎｋ进行登记，登记号分别为Ｕ８３２０８，Ｕ８３２７９，Ｕ８３３９７．此外还发现一批已知基因的表达与凋亡相关，其中包括Ｈｏｕ和人硫氧还原蛋白等，提示它们在凋亡中可能起作用．这项工作为进一步研究凋亡相关基因打下了良好基础．通过ＲＤＡ的研究结果，有可能发现人白血病细胞凋亡的特异标记蛋白或发挥作用的重要蛋白，以期为白血病治疗提供理论基础．The genes effecting in the process of the apoptosis of TF 1 cell line when it is deprived of the cytokine in the culture medium were studied by RDA (representational difference analysis) method. The TF 1 cell depriving of cytokines for 8 hours was selected as the Tester and normal cultured TF 1 cell as the Driver. Seven gene fragments were found uniquely expressed or highly expressed in the process of apoptosis of TF 1 cell line which include some known genes such as Hou and thioredoxin that formerly suggested to play a role in apoptosis.There are three fragments are complete novel after searching the nr and EST catalogues of GenBank and were banked into GenBank. The accession numbers for them are U83208, U83279, U83397 respectively. From the novel gene fragments, the complete cDNA sequence of them can be fished and the bioactivity and function of them in apoptosis of TF 1 cell and other hematological tumors can be further studied. On the other hand, the function of some known genes which were not suggested formerly in the course of apoptosis can be studied .

关 键 词：TF-1细胞株 代表差异分析 细胞凋亡 

分 类 号：Q28[生物学—细胞生物学] R329.25[医药卫生—人体解剖和组织胚胎学]