比色法测定Fenton反应产生的羟自由基及其应用  

Determination of Hydroxyl Radicals in Fenton Reaction by Colorimetric Assay and Its Application.

作　　者：徐向荣[1] 王文华[1] 李华斌[1] 

机构地区：[1]中国科学院生态环境研究中心

出　　处：《生物化学与生物物理进展》1999年第1期67-69,共3页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金

摘　　要：Ｆｅｎｔｏｎ反应产生的羟自由基与二甲亚砜反应，生成甲基亚磺酸，再与坚牢蓝ＢＢ盐反应生成偶氮砜，比色法测定其含量可间接测定ＯＨ·的生成量．通过对测定条件的研究，得到最佳实验方案．抗氧化剂药物硫脲和抗坏血酸与羟自由基清除率具有明显的量效关系．测定了核桃、黑芝麻等几种天然食物的水提取物清除羟自由基的功能．此法可用于羟自由基清除剂的筛选．Hydroxyl radicals produced in Fenton reaction can react with dimethyl sulfoxide to produce methane sulfinic acid which can further react with Fast Blue BB salt to form light yellow diazosulfone. According to the principle, hydroxyl radicals can be indirectly assayed by colorimetry. The determination condition was studied to obtain a best experimental program. The results showed that the hydroxyl radical scavenging effect were dose dependent upon thiourea and ascorbic acid. The walnut and black sesame were proved to be natural anti oxidation foods. The method can be applied to sieve the hydroxyl radical scavenger.

关 键 词：羟自由基 FENTON反应 比色法 甲基亚磺酸 

分 类 号：Q503[生物学—生物化学]