电针对局灶性脑缺血模型大鼠脑内STAT3阳性神经元的影响  

Effects of Electroacupuncture on STAT3 Positive Neurons in Brain of Focal Cerebral Ischemic Injury Rat

作　　者：付雪松[1] 于涛[2] 许能贵[3] 符文彬[2] 易玮[3] 包昆[2] 杨忠华[3] 

机构地区：[1]广州医学院从化学院,广东从化510925 [2]广州中医药大学第二临床医学院,广州510120 [3]广州中医药大学,广州510405

出　　处：《中西医结合研究》2010年第3期122-125,共4页Research of Integrated Traditional Chinese and Western Medicine

基　　金：广东省中医药管理局科技资助项目(No.2008179)

摘　　要：目的研究STAT3在脑缺血后的信号转导、调控机制以及电针的干预作用。方法采用热凝闭大脑中动脉建立大鼠局灶性脑缺血模型,采用免疫组织化学的方法检测不同时间段STAT3水平。结果相较假手术组,造模2h后STAT3阳性细胞数、平均截面积、平均光密度值出现轻微升高,差异无统计学意义(P>0.05),24h后明显升高,差异有统计学意义(P<0.05),3d后数值下降;电针组的检测结果与模型组的趋势相同,但各时间段在数值上均高于相应的模型组,差异有统计学意义(P<0.05)。结论电针对脑功能的改善作用,可能与其上调STAT3而减少病灶区神经细胞的凋亡有关。Objective To study STAT3 signal transduction,regulation and control mechanisms after cerebral ischemia and the role of electroacupuncture（EA）intervention. Methods Focal cerebral ischemia（FCI）model was established by middle cerebral artery occlusion （MCAO）with heat-coagulation method, and the levels of STAT3 positive neurons in the cortex and hippocampus CA1 area at various time periods were detected with immunohistochemical method. Results Compared to sham-operated group,the STAT3 positive cells,the average cross-sectional area, and the average optical density in the model group increased slightly after 2 h,and increased significantly after 24 h（P〈0.05）,each indicator declined after 3 days. The result of electro-acupuncture group had the same trend as the model group,but the data at various time periods increased significantly（P〈0.05）. Conclusion The improvement of brain function by EA therapy may be associated with increasing the STAT3 positive neurons and reducing apoptosis of the damaged neu.

关 键 词：电针 脑缺血 STAT3 

分 类 号：R245[医药卫生—针灸推拿学]