天然红松林等位酶研究  

ALLOZYME ANALYSIS ON NATURAL POPULATION FOR PINUS KORAIENSIS

作　　者：祖元刚[1] 张恒庆[1] 颜廷芬 袁晓颖 

机构地区：[1]东北林业大学森林植物生态学开放研究实验室

出　　处：《植物研究》1999年第1期75-79,共5页Bulletin of Botanical Research

基　　金：国家教委跨世纪优秀人才培养计划基金;黑龙江省省杰出青年科学基金

摘　　要：利用红松种子的单倍体胚乳，采用水平切片淀粉凝胶电泳技术，对采自三个地区的天然红松林的材料进行了１０种酶系统的等位酶实验，共获得了１８个等位基因位点，对这１８个位点等位基因的统计分析表明：红松种群的多态位点百分数为Ｐ＝７７．７８％，等位基因平均数为Ａ＝２．０，预期杂合度为Ｈｅ＝０．１６４８。红松种群间遗传分化程度在松属植物中属于较低水平（Ｇｓｔ＝５．３８％），在红松１８个等位基因位点的变异中，绝大部分变异９４．６２％来自种群内部。根据Ｎｅｉ方法计算的红松三个种群的遗传一致度和遗传距离为地理较近的小北湖与长白山地区种群的遗传距离最小（０．００９５），而地理距离最远的长白山种群与凉水种群的遗传距离（０．０１４３）却小于凉水种群与小北湖种群间的遗传距离（０．０１６６），表明了这三个种群在起源上的复杂性。Ten enzyme systems were tested for the endosperm of korean pine from three populations using the horizontal starch gel electrophoresis technique. 18 loci were obtained. The results indicated the ratio of polymorphic loci was 77.78%, the average number of alleles was 2.0(A) and the expected heterozygous was 0.1648(He). The genetic differentiation (Gst=5.38%) among populations was lower than other species in Pinus genus and most variations were within population. According to Nei's index, the genetic distance (0.0095) between Xiaobeihu and Changbai Mountain was nearest, not correlation to the geographical distance. It shows the original complexity of population.

关 键 词：红松 等位酶 遗传多样性 

分 类 号：Q949.665[生物学—植物学] S791.247[农业科学—林木遗传育种]