RAPD技术在玉米自交系亲缘关系研究中的应用  

Application of RAPD Analysis in the Study of Phylogenetic Relationship among Maize Inbred Lines

作　　者：孙致良[1] 张超良[2] 金德敏[2] 孙世孟[1] 郭宝太[1] 孙克莲 王斌[2] 

机构地区：[1]莱阳农学院,莱阳265200 [2]中国科学院遗传研究所,北京100101

出　　处：《Acta Genetica Sinica》1999年第1期61-68,共8页

基　　金：国家九五科技攻关项目!96-014-01-06(3);山东省农业厅重点科研项目!9562203C

摘　　要：通过对我国正在使用的 １２个玉米骨干自交系的 ＭＰＤ分析，从 ２２０个 Ｏｐｅｒｏｎ引物中筛选出１２个能产生稳定的遗传多态性的引物。利用这些引物扩增出的指纹图谱，进行聚类分析，可将全部供武自交系分成３个类群。第１类群包括黄早４系统的５个自交系；第２类群包括４７８和４８８两个姊妹系；第３类群包括５个关系较远的自交系，其中３个来自美国，１个是全部中国血统，１个既有美国血统又有中国血统。这个结果与根据各个自交系的已知系谱确立的亲缘关系基本一致。Twelve maize inbred lines were examined for RAPD analysis with 220 ten-mer Opron primers. Of them, 12 were selected for cluster analysis because they gave reproducible, polymorphic fingerprintings. Dendrogram constructed from RAPD data showed that twelve maize inbred lines were devided into three clusters. The first cluster consists of Huangzao-4 and four inbred lines whose one parent is Huangzao-4; 502-196 and 502 form the first subgroup since 502-196 is an improved inbred line of 502; Huangzao-4 and Wenhuang-31413 form the second subgroup; H21 forms the third subgroup because its another parent is an American inbred line. The second cluster includes two inbred lines, 478 and 488, which came from a same F2 plat. The five remaining inbred lines form a separate cluster for their different origin; Mol7, a well-known American inbred line, forms the first subgroup; 107 and 81 12, which also came from the United States, form the second subgroup; 340 and 515 form the third subgroup, 340 is a native inbred line whose parents are Chinese local varieties and 515 has American and Chinese genealogy. These results indicate that phylogenetic relationship determined by RAPD analysis is basically consistent with the pedigree of the twelve maize inbred lines.

关 键 词：玉米 自交系 RAPD标记 聚类分析 

分 类 号：S513.032[农业科学—作物学]