单室MFC型生物毒性传感器对重金属离子的检测研究  

Development of a Low-cost Single Chamber Microbial Fuel Cell Type BOD Sensor

作　　者：吴锋[1,2] 刘志[1] 周奔[3] 周顺桂[2] 饶力群[1] 王跃强[2] 

机构地区：[1]湖南农业大学生物科学与技术学院,长沙410128 [2]广东省生态环境与土壤研究所,广州510650 [3]广州市环境保护科学研究院,广州510620

出　　处：《环境科学》2010年第7期1596-1600,共5页Environmental Science

基　　金：国家高技术研究发展计划(863)项目(2009AA05Z115);广东省自然科学基金项目(07006759);广东省科学院科技创新引导项目(CX200704)

摘　　要：构建了单室微生物燃料电池(air-cathode microbial fuel cell,ACMFC)型生物毒性传感器,以含重金属离子(Cd2+、Cu2+)人工配水为检测对象,分析了有毒物质对检测仪的抑制率与有毒物质浓度的线性关系.结果表明,①单室MFC型生物毒性传感器结构简单,操作方便,灵敏度较高,可用于水体重金属离子(Cd2+、Cu2+)生物毒性的快速检测;②在实验条件下,该生物毒性传感器检测时间4h,清洗时间2～10min,恢复时间4h;③检测结果显示,实验室自配Cd2+、Cu2+及其混合液IC20值分别为0.6、0.8和0.25mg/L,有毒物质浓度与MFC产电量的抑制率呈显著正相关,相关系数分别为0.9960、0.9744和0.9907.The principle of the detector is based on the effect of microbial toxicity of water sample on the electricity generation in microbial fuel cell（MFC）.The performance of the MFC-type biotoxicity detector was evaluated with the synthetic water containing heavy metals of Cd2＋ and Cu2＋.The experimental results demonstrated that：① relative to the conventional methods,the MFC-type detector is easy to operate,and suitable for on-line measurements with high sensitivity;② it only requires 4 h to complete measurements,and can get ready for next measurement within 4 h;③ there is a significant linear correlation between the concentration of toxic metal（s） and inhibition ratios in Coulombic yields of MFC.As the IC20 （concentration causing 20% inhibition） of Cd2＋,Cu2＋ and mixed metals （Cd2＋ and Cu2＋） were 0.6,0.8 and 0.25 mg /L,the regression coefficients were shown to be 0.996 0,0.974 4 and 0.990 7.

关 键 词：微生物燃料电池 重金属 生物毒性 在线检测 生物传感器 

分 类 号：X830.2[环境科学与工程—环境工程]