疲劳性运动中线粒体电子漏引起质子漏增加  

Electron Leak Causes Proton Leak in Skeletal Muscle Mitochondria in Exercise induced Fatigue

作　　者：时庆德[1] 张勇[1] 陈家琦[1] 刘树森[2] 

机构地区：[1]天津运动医学研究所 [2]中国科学院动物研究所生物膜与膜生物工程国家重点实验室

出　　处：《生物化学与生物物理学报》1999年第1期97-100,共4页

基　　金：国家体委委管课题

摘　　要：以大鼠递增强度力竭性跑台运动为疲劳运动模型，观察了运动后大鼠骨骼肌线粒体电子漏和质子漏的变化。结果表明，运动性疲劳状态下大鼠骨骼肌线粒体超氧阴离子生成增加，脂质过氧化也显著增加，态４呼吸速率明显加快，呼吸控制比显著下降。结果提示，运动性疲劳状态下大鼠骨骼肌线粒体电子漏增加，线粒体质子漏增多是氧化磷酸化偶联程度下降的重要因素。实验结果支持电子漏引起质子漏的假说。Using an animal model of exercise induced fatigue by incremental exercise to exhaustion on a motor driven treadmill, the changes of the mitochondrial proton leak and electron leak in mitochondria of skeletal muscle from rats immediately after exhaustive exercise were observed. The results showed that, in exercise induced fatigue conditions, superoxide anions of rat skeletal muscle mitochondria increased;State 4 respiration rate significantly accelerated and respiration control ratio clearly decreased. Results show that, in the condition of exercise induced fatigue, the increased mitochondrial proton leak and electron leak may be important factors that cause the decrease in coupling of oxidative phosphorylation. Our results provide evidence supporting a hypothesis that electron leak causes proton leak in mitochondria.

关 键 词：线粒体 氧化磷酸化 运动性疲劳 电子漏 质子漏 

分 类 号：Q505[生物学—生物化学] G804.7[文化科学—运动人体科学]