鲍曼不动杆菌108株β-内酰胺酶检测及耐药分析  

Detection and drug resistance of β-lactamase in 108 strains of Acinetobacter baumanii

作　　者：郭普[1,2] 乔艳[3] 李峰[1] 郑晶[1] 沈继龙[2] 

机构地区：[1]蚌埠医学院第一附属医院检验科,安徽蚌埠233004 [2]安徽医科大学病原生物学教研室,安徽省病原生物学省级实验室,安徽省人兽共患病重点实验室,安徽合肥230032 [3]蚌埠医学院第一附属医院感染病科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2010年第6期624-626,共3页Journal of Bengbu Medical College

基　　金：蚌埠医学院课题基金资助项目(07024)

摘　　要：目的:分析鲍曼不动杆菌的医院感染分布特点、产β-内酰胺酶情况和耐药性。方法:对临床送检标本中分离出的108株引起医院感染的鲍曼不动杆菌分布及药敏结果进行回顾性分析,并分别采用硝基头孢噻吩纸片法、双纸片协同法、三维试验检测β-内酰胺酶、超广谱β-内酰胺酶(ESBLs)和头孢菌素酶。结果:鲍曼不动杆菌在重症监护治疗病房、神经外科的检出率分别为63.89%、11.11%;感染部位以呼吸道和手术切口为主,分别为77.78%和12.96%。该菌对临床常用抗生素有不同程度的耐药,发现多重耐药菌96株(88.89%)。108株鲍曼不动杆菌中共检出产ESBLs 11株,阳性率为10.19%;头孢菌素酶78株,阳性率为72.22%;产β-内酰胺酶阳性率为100.00%。结论:鲍曼不动杆菌是医院感染重要的条件致病菌,其对抗生素耐药率高,且多重耐药。产β-内酰胺酶为鲍曼不动杆菌耐药的主要原因之一。临床要重视合理使用抗生素,减少多重耐药菌株的产生。Objective：To investigate the clinical distribution,production and drug resistance of β-lactamase in nosocomial infection.Methods：The distribution and the drug resistance of 108 strains of Acinetobacter baumanii causing nosocomial infection were analyzed retrospectively.β-lactamase,cephalosporinase and extended spectrum β-lactamases（ESBLs）were detected by Nitrocefin test,3-dimension test and disc agar diffusion method,respectively.Results：The detection rates of Acinetobacter baumanii in ICU wards and neurosurgery wards were 63.89% and 11.11%,respectively.Acinetobacter baumanii was detected in 77.78% of the sputum samples and 12.96% of the secretion samples.The bacteria were resistant to most common antibiotics,and the rate of multiple-resistance was 88.89%.Eleven ESBLs bacteria and 78 cephalosporinase bacteria were detected in the 108 strains of Acinetobacter baumanii,accounting for 10.19% and 72.22%,respectively,and the positive rate of β-lactamase bacteria detected was 100.00%.Conclusions：Acinetobacter baumannii are the major opportunistic pathogenic bacteria in hospital infection.They are highly resistant to routine antimicrobial agents and even multi-drug resistant.The strains producing β-lactamase are the prominent cause of multi-drug resistance.Antimicrobial agents should be used rationally to decrease multi-drug resistance.

关 键 词：鲍曼不动杆菌 Β-内酰胺酶 耐药 

分 类 号：R378[医药卫生—病原生物学]