氧化苦参碱对HepG2.2.15细胞蛋白酪氨酸激酶活性的影响  

Affects of protein tyrosine kinase on HepG2.2.15 cell by oxymatrine

作　　者：聂红明[1] 汪蓉[1] 李运东[1] 金树根[1] 陈建杰[1] 高月求[1] 王灵台[1] 

机构地区：[1]上海中医药大学附属曙光医院,上海201203

出　　处：《中西医结合肝病杂志》2010年第3期161-163,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：上海市科委资助项目(No.04ZR14132);上海市卫生局资助项目(No.2006097);上海市高校创新团队建设项目(第1期)

摘　　要：目的:探讨氧化苦参碱抗乙肝病毒复制的作用机制。方法:采用HepG2.2.15细胞体外培养,以拉米夫定为对照药物,用ELISA方法检测细胞内总蛋白酪氨酸激酶活性,观察氧化苦参碱对细胞内蛋白酪氨酸激酶活性影响。结果:氧化苦参碱体外对HepG2.2.15细胞内总蛋白酪氨酸激酶抑制率为19.93%;而拉米夫定对蛋白酪氨酸激酶活性抑制率更高,达49.30%。结论:氧化苦参碱具有一定的抑制蛋白酪氨酸激酶活性作用,可能是其抗乙肝病毒复制的作用机制之一。Objective：To explore the mechanism of oxymatrine against the hepatitis B virus replication. Methods：HepG2.2.15 cell cultured in vitro,with lamivudine as control drugs,and used the ELISA method to detecte the activity of PTK,observed the effect of oxymatrine activity of PTK in cell.Results：Oxymatrine in vitro of the PTK inhibition rate was 19.93%,and lamivudine to inhibit PTK activity was higher up 49.30%.Conclusion：Oxymatrine has a certain effect of inhibiting the activity of PTK（protein tyrosine kinase）,and it may be one of the anti-hepatitis B virus replication mechanisms.

关 键 词：氧化苦参碱/药效学 蛋白酪氨酸激酶 HEPG2.2.15细胞 

分 类 号：R285[医药卫生—中药学]