补肝散含药血清对肝星状细胞的外周型苯二氮卓受体表达及凋亡的影响  

Effects of Bugansan medicated serum on hepatic stellate cells peripheral benzodiazpine receptor and HSC apoptosis

作　　者：汪厚祥 史华新[2] 陈盛铎[3] 

机构地区：[1]武汉市第十三医院,湖北武汉430100 [2]湖北中医药大学 [3]湖北省中医院

出　　处：《中西医结合肝病杂志》2010年第3期166-167,188,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：湖北省血防办公室资助项目(No.200414)

摘　　要：目的:探讨补肝散含药血清对培养肝星状细胞(HSC)的外周型苯二氮卓受体(PBR)及凋亡的影响,并从PBR角度探讨其作用机制。方法:在不同浓度(5%、10%、20%)补肝散含药血清液的作用下,在PBR高表达期的不同时间,采用流式细胞仪测定细胞凋亡指数,同时采用放射免疫法检测PBR特异性配基[3H]PK11195结合量。结果:与不含药血清组相比较,补肝散各含药血清组细胞凋亡指数明显增加,而HSC与[3H]PK11195结合量明显减少(P<0.05,P<0.01)。结论:补肝散含药血清可以促进HSC凋亡,可能通过PBR途径发挥作用。Objective：To explore Bugansan medicated serum on hepatic stellate cells（HSC） peripheral benzodiazepine receptor（PBR） and apoptosis,along with the discussion from PBR aspect.Methods： Under the treatment of different concentration （5%,10%,20%） Bugansan medicated serum in different stages of PBR high expression,apoptosis index was determined by FCM.And simultaneously radioimmunoassay was applied for detecting binding capacity of PBR specify ligand [3H]PK11195 to discuss the relation of cell apoptosi to PBR.Results： Compared with capacity of HSC membrane with [3H]PK11195 decreased with the increasing concentration of Bugansan medicated serum,there were statistical significance.Conclusion： Bugansan medicated serum can effect HSC in accelerating its apoptosis,maybe by PBR pathway.

关 键 词：补肝散含药血清 肝星状细胞 凋亡 外周型苯二氮卓受体 

分 类 号：R285.5[医药卫生—中药学]