青蒿素与蒿甲醚对人鼻咽癌CNE-1细胞放射增敏作用的比较研究  

Comparison of the radio-sensitizing effect between artemisinin and artemether on human nasopharyngeal carcinoma CNE-1 cell line

作　　者：潘麓羽[1] 曹建平[1] 陈光烈[1] 纪蓉[1] 封阳[1] 周媛媛[1] 彭晓梅[2] 崔丹[1] 朱巍[1] 樊赛军[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院放射医学与防护重点实验室,苏州215123 [2]江苏省吴江市疾病预防控制中心,吴江215200

出　　处：《辐射研究与辐射工艺学报》2010年第3期177-180,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金项目(30670632)资助

摘　　要：为了比较青蒿素(Artemisinin)和蒿甲醚(Artemether)对人鼻咽癌CNE-1细胞放射增敏作用的差异,取指数生长期的人鼻咽癌CNE-1细胞,采用细胞克隆形成法分别检测青蒿素和蒿甲醚对CNE-1细胞生长的抑制效应,比较两种药物抑制效应的差异性,并确定实验的药物浓度;将人鼻咽癌细胞分为对照组、单纯药物组、单纯照射组及联合治疗组。单纯药物组、联合治疗组分别分为青蒿素组和蒿甲醚亚组,射线照射剂量为0、2、4、6和8Gy,采用多靶单击数学模型拟合细胞存活曲线,取放射增敏比(SER)为达到相同生物效应时,单纯照射的剂量与联合治疗组的剂量之比。实验结果表明,两种药物对细胞的抑制作用均随着药物浓度的提高而增强,存在剂量依赖关系。青蒿素对CNE-1细胞的抑制作用高于蒿甲醚,测得青蒿素放射增敏比(SER)为1.272,蒿甲醚SER为1.481,表明蒿甲醚较青蒿素对人鼻咽癌CNE-1细胞具有更强的放射增敏作用。In order to compare the radiation sensitizing effect of artemisinin and artemether to human naso- pharyngeal carcinoma CNE-1 cell, the human nasopharyngeal carcinoma CNE-1 cell in index vegetal period was selected as experimental sample, and the cells clone-forming method was used to test growth inhibition of artemisini- nand and artemether to the CNE-1 cell, to estimate the difference of inhibitory effect between the two drugs, and to determine suitable drug concentration. The experimental samples were divided into control group, simple drug group, irradiated group and union treatment group with human nasopharyngeal carcinoma cells. The simple drug and union treatment group were also divided into artemisinin and artemether group, respectively. The exposed doses were set up as 0, 2, 4, 6, and 8Gy. The cell survival curve was fitted by an multi-target one-shot mathematic model and taking the radiation sensitizing enhancement ratio/SER （SER） equal to the dosage ratio of irradiated group and union treatment group when the same biological effect was achieved. It has been found that both drugs can enhance the inhibitory ef- fect with the increment of medicine concentration. The radiation sensitizing enhancement ratio/SER （SER） has been determined to be 1.272 and 1.481for artemisinin and artemether, respectively, which suggests that artemether has stronger radiation sensitizing effect compared with artemisinin in human nasopharyngeal carcinoma CNE-1 cells.

关 键 词：鼻咽癌细胞株 放射增敏 青蒿素 蒿甲醚 

分 类 号：Q691[生物学—生物物理学] R114[医药卫生—卫生毒理学]