鸡马立克病病毒强毒与疫苗毒PCR鉴别检测方法的建立  

Establishment of A PCR for Identifying the Virulent Strain and Attenuated Vaccine Strain of MDV

作　　者：汪德生[1,2] 罗明星[1] 史开志[1] 曾佑玲[1] 李永明[1,2] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州大学动物疫病研究所,贵州贵阳550025

出　　处：《动物医学进展》2010年第7期26-30,共5页Progress In Veterinary Medicine

基　　金：贵阳市农业局科学技术计划项目([2008]动防-01号)

摘　　要：在Ⅰ型马立克病病毒（MDV）基因组中针对132 bp串联重复序列的两侧合成一对引物,应用PCR技术对临床病例采集的疑似马立克病肿瘤病变鸡肝组织和1日龄接种CVI988弱毒疫苗的健康雏鸡羽髓样本进行检测。结果表明,从临床病例采集的10份肝脏组织,7份扩增出一条314 bp的条带,相当于2个拷贝数的132 bp串联重复序列;在接种疫苗健康雏鸡的羽髓样本中,扩增出与CVI988弱毒一致的PCR图谱,相当于6个~8个或更多拷贝数的132 bp串联重复序列。根据PCR图谱的差异即可鉴别MDV强毒株与CVI988疫苗弱毒株。A pair of primers were synthesized to flank the 132 bpr tandem direct repeats in the serotype 1 of Marek＇s disease virus（MDV） genome.The polymerase chain reaction（PCR） was applied to detect the liver materials of suspicious MDV affected chickens in clinical cases and the feather pulp samples of healthy chicks inoculated with CVI988 vaccine at 1 day age.The results showed that out of 10 liver materials,7 amplified a 314bp DNA fragment,corresponding to two copies of the 132bpr tandem direct repeats.The PCR spectrum was in accordance with CVI988 attenuated vaccine,when the feather pulp samples were PCR-amplified,corresponding to 6-8 or more copies of the 132bpr tandem direct repeats.According to the discrepancy of PCR spectrum,the virulent MDV strain could be differentiated from CVI988 attenuated vaccine strain.The feather pulps were collected as the samples and the efficacy of chicks inoculated with serotype 1 vaccine was also surveyed in this experiment.

关 键 词：马立克病病毒 CVI988弱毒疫苗 PCR 

分 类 号：Q789[生物学—分子生物学] S852.659.1[农业科学—基础兽医学]